Institute for Limnology, Austrian Academy of Sciences, Mondseestrasse 9, A-5310 Mondsee, Austria.
Faculty of Science, University of South Bohemia, Branišovská 31, 37005 České Budějovice, Czech Republic.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2946-2950. doi: 10.1099/ijs.0.022384-0. Epub 2010 Jan 29.
A chemo-organotrophic, aerobic, non-motile strain, MWH-BRAZ-DAM2D(T), isolated from a freshwater pond in Brazil, was characterized phenotypically, phylogenetically and chemotaxonomically. Phylogenetic analysis of 16S rRNA gene sequences indicated affiliation of the strain with the genus Limnohabitans (Comamonadaceae, Betaproteobacteria). 16S rRNA gene sequence similarities between the isolate and Limnohabitans curvus MWH-C5(T), representing the type species of the genus, and the type strains of Limnohabitans parvus and Limnohabitans planktonicus were 98.2, 96.5 and 97.0 %, respectively. DNA-DNA reassociation analyses with DNA of the type strains of all three previously described Limnohabitans species revealed similarity values in the range 26.2-44.6 %. The predominant fatty acids of the isolate were C(16 : 1)ω7c/ω6c, C(16 : 0), C(12 : 0) and C(8 : 0) 3-OH, the major quinone was ubiquinone Q-8 and the DNA G+C content was 55.8 mol%. The isolate could be discriminated from the type strains of the three Limnohabitans species by several phenotypic traits including differences in the utilization of several carbon sources. Based on the phylogeny of the isolate and its differences from the three most closely related species, the isolate represents a novel species for which the name Limnohabitans australis sp. nov. is proposed. The type strain is MWH-BRAZ-DAM2D(T) (=DSM 21646(T)=CCUG 56719(T)).
一株从巴西淡水池塘中分离得到的嗜碳、需氧、非运动型菌株 MWH-BRAZ-DAM2D(T)，在表型、系统发育和化学分类学上进行了特征描述。16S rRNA 基因序列的系统发育分析表明，该菌株与 Limnohabitans 属（Comamonadaceae，Betaproteobacteria）有关。该分离株与 Limnohabitans curvus MWH-C5(T)（代表该属的模式种）和 Limnohabitans parvus 和 Limnohabitans planktonicus 的模式株的 16S rRNA 基因序列相似性分别为 98.2%、96.5%和 97.0%。与所有三种先前描述的 Limnohabitans 种的模式株的 DNA-DNA 同源性分析显示相似性值在 26.2-44.6%之间。该分离株的主要脂肪酸为 C(16 : 1)ω7c/ω6c、C(16 : 0)、C(12 : 0)和 C(8 : 0) 3-OH，主要醌为 ubiquinone Q-8，DNA G+C 含量为 55.8 mol%。该分离株可以通过几种表型特征与三种 Limnohabitans 种的模式株区分开来，包括对几种碳源利用的差异。基于该分离株的系统发育及其与三种最密切相关的种的差异，该分离株代表了一个新种，建议命名为 Limnohabitans australis sp. nov.。模式株为 MWH-BRAZ-DAM2D(T)（=DSM 21646(T)=CCUG 56719(T)）。
