NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan.
Appl Environ Microbiol. 2010 Mar;76(6):1783-8. doi: 10.1128/AEM.00668-09. Epub 2010 Jan 29.
Microbiologically influenced corrosion of steel in anaerobic environments has been attributed to hydrogenotrophic microorganisms. A sludge sample collected from the bottom plate of a crude-oil storage tank was used to inoculate a medium containing iron (Fe(0)) granules, which was then incubated anaerobically at 37 degrees C under an N(2)-CO(2) atmosphere to enrich for microorganisms capable of using iron as the sole source of electrons. A methanogen, designated strain KA1, was isolated from the enrichment culture. An analysis of its 16S rRNA gene sequence revealed that strain KA1 is a Methanococcus maripaludis strain. Strain KA1 produced methane and oxidized iron much faster than did the type strain of M. maripaludis, strain JJ(T), which produced methane at a rate expected from the abiotic H(2) production rate from iron. Scanning electron micrographs of iron coupons that had been immersed in either a KA1 culture, a JJ(T) culture, or an aseptic medium showed that only coupons from the KA1 culture had corroded substantially, and these were covered with crystalline deposits that consisted mainly of FeCO(3).
在厌氧环境中，微生物影响的钢腐蚀归因于氢营养型微生物。从原油储罐底板采集的污泥样品被用来接种含有铁（Fe(0)）颗粒的培养基，然后在 37 摄氏度下在 N(2)-CO(2)气氛中进行厌氧孵育，以富集能够将铁作为唯一电子来源的微生物。从富集培养物中分离出一种被命名为 KA1 的产甲烷菌。对其 16S rRNA 基因序列的分析表明，KA1 菌株是一种 Methanococcus maripaludis 菌株。与 Methanococcus maripaludis 的 JJ(T) 型菌株相比，KA1 菌株产生甲烷和氧化铁的速度要快得多，而 JJ(T) 型菌株产生甲烷的速度与铁的非生物 H(2)产生速率相当。浸泡在 KA1 培养物、JJ(T)培养物或无菌培养基中的铁试片的扫描电子显微镜图像显示，只有来自 KA1 培养物的试片发生了实质性腐蚀，并且这些试片表面覆盖着主要由 FeCO(3)组成的结晶沉积物。
