Jeanthon C, L'Haridon S, Reysenbach A L, Vernet M, Messner P, Sleytr U B, Prieur D
Station Biologique, UPR9042, Central National de la Recherche Scientifique, Roscoff, France.
Int J Syst Bacteriol. 1998 Jul;48 Pt 3:913-9. doi: 10.1099/00207713-48-3-913.
An autotrophic, extremely thermophilic methanogen (ME(T)) was isolated from a deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge at a depth of 3000 m. The heavily flagellated cells are motile and coccoid shaped. The new strain growths between 55 and 91 degrees C, with an optimum growth temperature at 85 degree C. The optimum pH for growth is 6.5, and the optimum sea salt concentration for growth is around 25 g l-1. The organism uses H2 and CO2 as the only substrate for growth and methane production. Tungsten, selenium and yeast extract stimulate growth significantly. In the presence of CO2 and H2, the organism reduces elemental sulphur to hydrogen sulphide. The G+C content of the genomic DNA is 33 mol%. As determined by 16S gene sequence analysis, this organism is closely related to Methanococcus jannaschii strain JAL-1T. However, no significant homology was observed between them with DNA-DNA hybridization. It is proposed that this organism should be placed in a new species, Methanococcus infernus. The type strain is ME(T) (= DSM 11812T).
从大西洋中脊3000米深处采集的深海热液烟囱样本中分离出一种自养、极端嗜热的产甲烷菌（ME(T)）。该菌细胞有大量鞭毛，具运动性，呈球状。新菌株在55至91摄氏度之间生长，最适生长温度为85摄氏度。生长的最适pH值为6.5，最适海盐浓度约为25 g l-1。该生物体利用氢气和二氧化碳作为生长和产甲烷的唯一底物。钨、硒和酵母提取物能显著刺激生长。在二氧化碳和氢气存在的情况下，该生物体将元素硫还原为硫化氢。基因组DNA的G+C含量为33 mol%。通过16S基因序列分析确定，该生物体与詹氏甲烷球菌菌株JAL-1T密切相关。然而，通过DNA-DNA杂交未观察到它们之间有显著同源性。建议将该生物体归入一个新物种，即地狱甲烷球菌。模式菌株为ME(T)（= DSM 11812T）。
