无义介导的 mRNA 去帽反应发生在酿酒酵母的多核糖体上。
Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae.
机构信息
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA.
出版信息
Nat Struct Mol Biol. 2010 Feb;17(2):244-7. doi: 10.1038/nsmb.1734. Epub 2010 Jan 31.
Abstract
Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5' end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.
摘要
无意义介导的衰变 (NMD) 降解含有过早翻译终止密码子的 mRNA。在酵母中，NMD 底物从 5' 端被脱帽并进行外切核酸酶消化。尽管在识别中需要翻译，但含有无意义的 mRNA 的降解被认为发生在核糖体游离的细胞质 P 体中。我们发现脱帽的含有无意义的 mRNA 与多核糖体结合，这表明识别和降解紧密偶联，并且多核糖体是异常 mRNA 降解的主要部位。
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