Laboratoire d'Aquaculture, Institut National des Sciences et Technologies de la Mer BP59, route de Khniss, 5000, Monastir, Tunisia.
Environ Monit Assess. 2010 Dec;171(1-4):661-9. doi: 10.1007/s10661-010-1311-0. Epub 2010 Jan 30.
The toxicity of mercury, zinc and copper on sperm viability, fertilisation and embryogenesis of Ruditapes decussatus was examined. Cu did not affect sperm viability at all the concentrations tested. Conversely, the Zn and Hg significantly (P<0.01) reduced sperm viability only at the highest concentration (respectively 512 and 256 μg/l). Cu caused a significant decrease (p<0.05) of less than 6% in the fertilisation rate at 128 μg/l and Zn of up to 13% at 64 μg/l. Hg significantly (p<0.01) inhibited fertilisation at concentrations as low as 32 μg/l. The median effective concentrations (EC50) reducing rates of embryogenesis by 50% were 21.1 μg Hg/l (0.1 μM), 46.3 μg Cu/l (0.72 μM) and 43.4 μg Zn/l. Therefore, Hg is up to seven times more toxic than Cu (on a molar basis). Ecotoxicity of mercury on larvae survival was also assessed in this work. Result showed a significant (p<0.05) reduction of survival after exposure to 4 and 12 μg/l of Hg. The fertilisation rate and embryogenesis were the most sensitive endpoints, although the latter is more advisable for routine assessment of seawater quality because of its greater sensibility.
研究了汞、锌和铜对菲律宾蛤仔（Ruditapes decussatus）精子活力、受精和胚胎发生的毒性。Cu 在所有测试浓度下均未影响精子活力。相反，Zn 和 Hg 仅在最高浓度（分别为 512 和 256μg/l）下显著（P<0.01）降低精子活力。Cu 在 128μg/l 时导致受精率显著降低（p<0.05），低于 6%，而 Zn 在 64μg/l 时则降低了高达 13%。Hg 在浓度低至 32μg/l 时就显著（p<0.01）抑制了受精。将胚胎发生率降低 50%的半数有效浓度（EC50）分别为 21.1μg Hg/l（0.1μM）、46.3μg Cu/l（0.72μM）和 43.4μg Zn/l。因此，Hg 的毒性比 Cu 高（基于摩尔）高达七倍。本工作还评估了汞对幼虫存活率的生态毒性。结果表明，暴露于 4 和 12μg/l 的 Hg 后，存活率显著降低（p<0.05）。受精率和胚胎发生是最敏感的终点，尽管后者更适合常规评估海水质量，因为其更灵敏。
