Eida Amany M, Eida Mohamed M, El-Desoky Amina
Department of Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
J Egypt Soc Parasitol. 2009 Dec;39(3):975-90.
The genotyping of cryptosporidium clinical isolates obtained from 36 gastrointestinal symptomatic patients were identified by nested PCR for amplification of 18S rRNA followed by RFLP analysis using Ssp1 and Vsp1, and then pathological changes between different cryptosporidium genotypes were evaluated in experimentally infected mice. Cryptosporidium genotypes (C. parvum, C. hominis & C. melegridies) were detected (66.7%, 27.7% & 5.6%) respectively in human isolates. Different degrees of pathological changes were found among infected mice by different Cryptosporidium genotypes. Moderate and severe degrees of pathological changes with infection score ranging from 2 to 4 were found in all infected mice with C. parvum (except one isolate), while mild degree of pathological changes with infection score of 2 was found in all mice with C. melegridies. The results showed statistically significant relation between genotype and pathological degrees. There were no differences in the average number of oocysts per smear in Group II and Group III while in Group I, there was no oocysts shedding.
对36例有胃肠道症状患者的隐孢子虫临床分离株进行基因分型，采用巢式PCR扩增18S rRNA，随后用Ssp1和Vsp1进行限制性片段长度多态性（RFLP）分析，然后在实验感染的小鼠中评估不同隐孢子虫基因型之间的病理变化。在人类分离株中分别检测到隐孢子虫基因型（微小隐孢子虫、人隐孢子虫和火鸡隐孢子虫）（66.7%、27.7%和5.6%）。不同隐孢子虫基因型感染的小鼠出现了不同程度的病理变化。所有感染微小隐孢子虫的小鼠（除一个分离株外）均出现中度和重度病理变化，感染评分为2至4，而所有感染火鸡隐孢子虫的小鼠均出现轻度病理变化，感染评分为2。结果显示基因型与病理程度之间存在统计学上的显著关系。第二组和第三组每涂片卵囊平均数量无差异，而第一组无卵囊排出。
