The David H. Koch Institute for Integrative Cancer Research, Massachusetts Instituteof Technology, Cambridge, MA 02139, USA.
ACS Nano. 2010 Feb 23;4(2):625-31. doi: 10.1021/nn901319y.
The targeted delivery of therapeutics to tumors remains an important challenge in cancer nanomedicine. Attaching nanoparticles to cells that have tumoritropic migratory properties is a promising modality to address this challenge. Here we describe a technique to create nanoparticulate cellular patches that remain attached to the membrane of cells for up to 2 days. NeutrAvidin-coated nanoparticles were anchored on cells possessing biotinylated plasma membrane. Human bone marrow derived mesenchymal stem cells with nanoparticulate patches retained their inherent tumoritropic properties as shown using a tumor model in a 3D extracellular matrix. Additionally, human umbilical vein endothelial cells with nanoparticulate patches were able to retain their functional properties and form multicellular structures as rapidly as unmodified endothelial cells. These results provide a novel strategy to actively deliver nanostructures and therapeutics to tumors utilizing stem cells as carriers and also suggest that nanoparticulate cellular patches may have applications in tissue regeneration.
将治疗药物靶向递送至肿瘤仍然是癌症纳米医学的一个重要挑战。将纳米颗粒附着在具有肿瘤趋向性迁移特性的细胞上，是解决这一挑战的一种很有前途的方式。在这里，我们描述了一种创建纳米颗粒细胞贴附物的技术，该贴附物可以在细胞表面保持附着长达 2 天。带正电荷的纳米颗粒通过静电吸附作用被锚定在带有生物素化质膜的细胞上。带有纳米颗粒贴附物的人骨髓间充质干细胞保留了其固有的肿瘤趋向性特性，这在 3D 细胞外基质中的肿瘤模型中得到了证实。此外，带有纳米颗粒贴附物的人脐静脉内皮细胞能够像未修饰的内皮细胞一样快速保留其功能特性并形成多细胞结构。这些结果为利用干细胞作为载体将纳米结构和治疗药物主动递送至肿瘤提供了一种新的策略，同时也表明纳米颗粒细胞贴附物可能在组织再生方面有应用前景。
