phd/doc操纵子抗毒素Phd的纯化与结晶
Purification and crystallization of Phd, the antitoxin of the phd/doc operon.
作者信息
Garcia-Pino Abel, Sterckx Yann, Vandenbussche Guy, Loris Remy
机构信息
Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):167-71. doi: 10.1107/S1744309109051550. Epub 2010 Jan 27.
Abstract
The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.
摘要
来自噬菌体P1的phd/doc模块的抗毒素Phd以两种不同的晶体形式结晶。带有His标签的Phd晶体包含该蛋白质的C末端截短版本，衍射分辨率为2.20 Å。从Phd-Doc复合物中纯化的未标记Phd晶体衍射分辨率为2.25 Å。这些晶体部分呈半面体孪晶，包含该蛋白质的全长版本。
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