拟南芥重组VSP1的结晶及初步晶体学分析
Crystallization and preliminary crystallographic analysis of recombinant VSP1 from Arabidopsis thaliana.
作者信息
Shi Zhu-Bing, Ge Hong-Hua, Zhao Ping, Zhang Min
机构信息
School of Life Science, Anhui University, Hefei 230039, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):201-3. doi: 10.1107/S1744309109053688. Epub 2010 Jan 28.
Abstract
VSP1 is a defence protein in Arabidopsis thaliana that may also be involved in control of plant development. The recombinant protein has been overexpressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method. The crystal diffracted to 1.9 A resolution and a complete X-ray data set was collected at 100 K using Cu Kalpha radiation from a rotating-anode X-ray source. The crystals belonged to space group C2. As there are no related structures that could be used as a search model for molecular replacement, work is in progress on experimental phasing using heavy-atom derivatives and selenomethionine derivatives.
摘要
VSP1是拟南芥中的一种防御蛋白，它可能也参与植物发育的调控。该重组蛋白已在大肠杆菌中过表达，经纯化后采用坐滴气相扩散法进行结晶。晶体衍射分辨率达到1.9埃埃，使用旋转阳极X射线源的Cu Kα辐射在100 K下收集了完整的X射线数据集。晶体属于空间群C2。由于没有相关结构可作为分子置换的搜索模型，目前正在使用重原子衍生物和硒代甲硫氨酸衍生物进行实验相位分析。
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