Seo Kyung Hye, Zhuang Ningning, Cha Joon-Yung, Son Daeyoung, Lee Kon Ho
Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju 660-701, Republic of Korea.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 1;68(Pt 1):101-4. doi: 10.1107/S1744309111050597. Epub 2011 Dec 24.
A DJ-1 homologue protein from Arabidopsis thaliana (AtDJ-1D) belongs to the DJ-1/ThiJ/Pfpl superfamily and contains two tandem arrays of DJ-1-like sequences, but no structural information is available to date for this protein. AtDJ-1D was expressed in Escherichia coli, purified and crystallized for structural analysis. A crystal of AtDJ-1D was obtained by the hanging-drop vapour-diffusion method using 0.22 M NaCl, 0.1 M bis-tris pH 6.5, 21% polyethylene glycol 3350. AtDJ-1D crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 56.78, b = 75.21, c = 141.68 Å, β = 96.87°, and contained a trimer in the asymmetric unit. Diffraction data were collected to 2.05 Å resolution. The structure of AtDJ-1D has been determined using the multiple-wavelength anomalous dispersion (MAD) method.
来自拟南芥的一种DJ-1同源蛋白（AtDJ-1D）属于DJ-1/ThiJ/Pfpl超家族，包含两个串联排列的类DJ-1序列，但迄今为止尚无该蛋白的结构信息。AtDJ-1D在大肠杆菌中表达、纯化并结晶以进行结构分析。通过悬滴气相扩散法，使用0.22 M NaCl、0.1 M 双三羟甲基氨基甲烷pH 6.5、21%聚乙二醇3350获得了AtDJ-1D晶体。AtDJ-1D晶体属于单斜空间群P2(1)，晶胞参数a = 56.78、b = 75.21、c = 141.68 Å，β = 96.87°，不对称单元中包含一个三聚体。收集到了分辨率为2.05 Å的衍射数据。已使用多波长反常散射（MAD）方法确定了AtDJ-1D的结构。
