来自拟南芥的DJ-1同源物（AtDJ-1D）的结晶及初步X射线数据分析。
Crystallization and preliminary X-ray data analysis of a DJ-1 homologue from Arabidopsis thaliana (AtDJ-1D).
作者信息
Seo Kyung Hye, Zhuang Ningning, Cha Joon-Yung, Son Daeyoung, Lee Kon Ho
机构信息
Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju 660-701, Republic of Korea.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 1;68(Pt 1):101-4. doi: 10.1107/S1744309111050597. Epub 2011 Dec 24.
Abstract
A DJ-1 homologue protein from Arabidopsis thaliana (AtDJ-1D) belongs to the DJ-1/ThiJ/Pfpl superfamily and contains two tandem arrays of DJ-1-like sequences, but no structural information is available to date for this protein. AtDJ-1D was expressed in Escherichia coli, purified and crystallized for structural analysis. A crystal of AtDJ-1D was obtained by the hanging-drop vapour-diffusion method using 0.22 M NaCl, 0.1 M bis-tris pH 6.5, 21% polyethylene glycol 3350. AtDJ-1D crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 56.78, b = 75.21, c = 141.68 Å, β = 96.87°, and contained a trimer in the asymmetric unit. Diffraction data were collected to 2.05 Å resolution. The structure of AtDJ-1D has been determined using the multiple-wavelength anomalous dispersion (MAD) method.
摘要
来自拟南芥的一种DJ-1同源蛋白（AtDJ-1D）属于DJ-1/ThiJ/Pfpl超家族，包含两个串联排列的类DJ-1序列，但迄今为止尚无该蛋白的结构信息。AtDJ-1D在大肠杆菌中表达、纯化并结晶以进行结构分析。通过悬滴气相扩散法，使用0.22 M NaCl、0.1 M 双三羟甲基氨基甲烷pH 6.5、21%聚乙二醇3350获得了AtDJ-1D晶体。AtDJ-1D晶体属于单斜空间群P2(1)，晶胞参数a = 56.78、b = 75.21、c = 141.68 Å，β = 96.87°，不对称单元中包含一个三聚体。收集到了分辨率为2.05 Å的衍射数据。已使用多波长反常散射（MAD）方法确定了AtDJ-1D的结构。
相似文献
1
Crystallization and preliminary X-ray data analysis of a DJ-1 homologue from Arabidopsis thaliana (AtDJ-1D).来自拟南芥的DJ-1同源物（AtDJ-1D）的结晶及初步X射线数据分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 1;68(Pt 1):101-4. doi: 10.1107/S1744309111050597. Epub 2011 Dec 24.
2
Crystallization and preliminary X-ray characterization of a novel calcium-binding protein AtCBL2 from Arabidopsis thaliana.来自拟南芥的一种新型钙结合蛋白AtCBL2的结晶及初步X射线表征
Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1079-80. doi: 10.1107/s0907444903007029. Epub 2003 May 23.
3
Crystallization and preliminary X-ray analysis of tubulin-folding cofactor A from Arabidopsis thaliana.拟南芥微管蛋白折叠辅助因子A的结晶及初步X射线分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt 8):954-6. doi: 10.1107/S1744309110023900. Epub 2010 Jul 29.
4
Expression, purification, crystallization and preliminary X-ray diffraction analysis of Arabidopsis thaliana cyclophilin 38 (AtCyp38).拟南芥亲环蛋白38（AtCyp38）的表达、纯化、结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Dec 1;61(Pt 12):1087-9. doi: 10.1107/S1744309105037681. Epub 2005 Nov 24.
5
Crystallization and preliminary crystallographic analysis of a C2 protein from Arabidopsis thaliana.拟南芥C2蛋白的结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1575-8. doi: 10.1107/S1744309111040541. Epub 2011 Nov 26.
6
Crystallization and preliminary crystallographic analysis of recombinant VSP1 from Arabidopsis thaliana.拟南芥重组VSP1的结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):201-3. doi: 10.1107/S1744309109053688. Epub 2010 Jan 28.
7
Crystallization and preliminary X-ray analysis of 4-coumarate:CoA ligase from Arabidopsis thaliana.来自拟南芥的4-香豆酸:辅酶A连接酶的结晶及初步X射线分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt 3):409-11. doi: 10.1107/S1744309111002181. Epub 2011 Feb 25.
8
Recombinant ACHT1 from Arabidopsis thaliana: crystallization and X-ray crystallographic analysis.来自拟南芥的重组ACHT1：结晶及X射线晶体学分析
Acta Crystallogr F Struct Biol Commun. 2017 Jul 1;73(Pt 7):382-385. doi: 10.1107/S2053230X17007725. Epub 2017 Jun 17.
9
The complex between SOS3 and SOS2 regulatory domain from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis.拟南芥中SOS3与SOS2调节结构域的复合物：克隆、表达、纯化、结晶及初步X射线分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt 7):568-70. doi: 10.1107/S174430910702533X. Epub 2007 Jun 11.
10
Purification, crystallization and preliminary X-ray diffraction analysis of S-formylglutathione hydrolase from Arabidopsis thaliana: effects of pressure and selenomethionine substitution on space-group changes.拟南芥S-甲酰谷胱甘肽水解酶的纯化、结晶及初步X射线衍射分析：压力和硒代甲硫氨酸取代对空间群变化的影响
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2272-4. doi: 10.1107/s0907444903020031. Epub 2003 Nov 27.
引用本文的文献
1
Control of heat and oxidative stress adaptation by the DJ-1 paralogs in Arabidopsis thaliana.拟南芥中DJ-1同源物对热和氧化应激适应的调控
Plant Mol Biol. 2025 Aug 21;115(5):105. doi: 10.1007/s11103-025-01630-5.
2
Unusual DNA-binding properties of the Arabidopsis thaliana WRKY50 transcription factor at target gene promoters.拟南芥WRKY50转录因子在靶基因启动子处的异常DNA结合特性。
Plant Cell Rep. 2021 Jan;40(1):69-83. doi: 10.1007/s00299-020-02611-2. Epub 2020 Oct 2.
3
Oxidative stress triggers aggregation of GFP-tagged Hsp31p, the budding yeast environmental stress response chaperone, and glyoxalase III.氧化应激引发 GFP 标记的 Hsp31p（芽殖酵母环境应激反应伴侣蛋白）和甘油醛-3-磷酸脱氢酶 III 的聚集。
Cell Stress Chaperones. 2018 Jul;23(4):595-607. doi: 10.1007/s12192-017-0868-8. Epub 2017 Dec 20.
4
Functional dissection of a strong and specific microbe-associated molecular pattern-responsive synthetic promoter.一种强效且特异性的微生物相关分子模式响应性合成启动子的功能剖析
Plant Biotechnol J. 2016 Jan;14(1):61-71. doi: 10.1111/pbi.12357. Epub 2015 Mar 27.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
A plant DJ-1 homolog is essential for Arabidopsis thaliana chloroplast development.一种植物 DJ-1 同源物对于拟南芥叶绿体发育是必需的。
PLoS One. 2011;6(8):e23731. doi: 10.1371/journal.pone.0023731. Epub 2011 Aug 23.
3
The Arabidopsis DJ-1a protein confers stress protection through cytosolic SOD activation.拟南芥 DJ-1a 蛋白通过细胞溶质 SOD 的激活赋予应激保护。
J Cell Sci. 2010 May 15;123(Pt 10):1644-51. doi: 10.1242/jcs.063222. Epub 2010 Apr 20.
4
Involvement of ERK1/2 signaling pathway in DJ-1-induced neuroprotection against oxidative stress.ERK1/2信号通路参与DJ-1诱导的针对氧化应激的神经保护作用。
Biochem Biophys Res Commun. 2009 Jun 12;383(4):469-74. doi: 10.1016/j.bbrc.2009.04.037. Epub 2009 Apr 14.
5
DJ-1 modulates UV-induced oxidative stress signaling through the suppression of MEKK1 and cell death.DJ-1 通过抑制 MEKK1 和细胞死亡来调节紫外线诱导的氧化应激信号通路。
Cell Death Differ. 2008 Jun;15(6):1030-41. doi: 10.1038/cdd.2008.26. Epub 2008 Feb 29.
6
DJ-1 binds androgen receptor directly and mediates its activity in hormonally treated prostate cancer cells.DJ-1直接结合雄激素受体并在激素处理的前列腺癌细胞中介导其活性。
Cancer Res. 2007 May 15;67(10):4630-7. doi: 10.1158/0008-5472.CAN-06-4556.
7
Identification of functional subclasses in the DJ-1 superfamily proteins.DJ-1超家族蛋白中功能亚类的鉴定。
PLoS Comput Biol. 2007 Jan 26;3(1):e10. doi: 10.1371/journal.pcbi.0030010.
8
Mutational analysis of DJ-1 in Drosophila implicates functional inactivation by oxidative damage and aging.果蝇中DJ-1的突变分析表明其功能因氧化损伤和衰老而失活。
Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12517-22. doi: 10.1073/pnas.0601891103. Epub 2006 Aug 7.
9
Drosophila DJ-1 mutants show oxidative stress-sensitive locomotive dysfunction.果蝇DJ-1突变体表现出对氧化应激敏感的运动功能障碍。
Gene. 2005 Nov 21;361:133-9. doi: 10.1016/j.gene.2005.06.040. Epub 2005 Oct 3.
10
The atomic resolution crystal structure of the YajL (ThiJ) protein from Escherichia coli: a close prokaryotic homologue of the Parkinsonism-associated protein DJ-1.来自大肠杆菌的YajL（ThiJ）蛋白的原子分辨率晶体结构：帕金森病相关蛋白DJ-1的紧密原核同源物。
J Mol Biol. 2005 Oct 28;353(3):678-91. doi: 10.1016/j.jmb.2005.08.033. Epub 2005 Sep 2.
Zotero 插件