Atkins D, Ibbotson K J, Hillier K, Hunt N H, Hammonds J C, Martin T J
Br J Cancer. 1977 Nov;36(5):601-7. doi: 10.1038/bjc.1977.237.
Fragments of human renal carcinoma tissue have been co-cultured with mouse calvaria. In 9/13 cases significant bone resorption occurred whilst in no case did control kidney cause significant resorption. When bone resorption did occur, it could be reduced by inclusion of indomethacin in the culture medium. In some cases when theophylline was included in culture medium to prevent cyclic AMP breakdown, there was enhancement of tumour-induced bone resorption. Control studies without tumour showed that none of the experimental treatments had a direct effect on bone. Radioimmunoassay of prostaglandin E (PGE) levels in pooled culture media showed that tumour fragments produced appreciable amounts of PGE, and that this production was lowered by indomethacin and increased by theophylline. It is concluded that the bone resorption induced by these tumours is due to a prostaglandin, and that prostaglandin production may be controlled by changes in cyclic AMP metabolism.
人肾癌组织碎片已与小鼠颅骨共同培养。在13例中有9例出现了显著的骨吸收，而对照肾在任何情况下均未引起显著的骨吸收。当发生骨吸收时，通过在培养基中加入消炎痛可使其减轻。在某些情况下，当在培养基中加入茶碱以防止环磷腺苷（cAMP）分解时，肿瘤诱导的骨吸收增强。无肿瘤的对照研究表明，所有实验处理对骨均无直接影响。对混合培养基中前列腺素E（PGE）水平的放射免疫分析表明，肿瘤碎片产生了相当数量的PGE，消炎痛可降低其产生量，而茶碱可增加其产生量。得出的结论是，这些肿瘤诱导的骨吸收是由一种前列腺素引起的，并且前列腺素的产生可能受环磷腺苷代谢变化的控制。
