Department of Microbiology, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
J Microbiol. 2009 Dec;47(6):796-800. doi: 10.1007/s12275-009-0329-x. Epub 2010 Feb 4.
This study determined the complete nucleotide sequence of the plasmid pSY3 from Sphingobium chungbukense DJ77. It was 35,735 bp long with a G+C content of 61.9%. Forty open reading frames (ORFs) were found. We predicted these ORFs would encode proteins associated with plasmid replication, conjugative transfer, transposition of genes, plasmid stability/partition, hypothetical protein, and some other functions. Genes for biodegradation were not found. No other plasmid homologous to pSY3 in the overall nucleotide sequence or gene organization could be found in the NCBI database.
本研究确定了从 Sphingobium chungbukense DJ77 中分离得到的质粒 pSY3 的完整核苷酸序列。该质粒长 35735bp,GC 含量为 61.9%。共发现 40 个开放阅读框(ORFs)。我们预测这些 ORFs 编码与质粒复制、共轭转移、基因转位、质粒稳定性/分区、假设蛋白和其他一些功能相关的蛋白。未发现与生物降解相关的基因。在 NCBI 数据库中,没有发现与 pSY3 在整体核苷酸序列或基因组织上同源的其他质粒。