Mazoyon Candice, Hirel Bertrand, Pecourt Audrey, Catterou Manuella, Gutierrez Laurent, Sarazin Vivien, Dubois Fréderic, Duclercq Jérôme
Unité Ecologie et Dynamique des Systèmes Anthropisés (EDYSAN, UMR7058 CNRS), Université de Picardie Jules Verne (UPJV), 80000 Amiens, France.
Unité Mixte de Recherche 1318 INRA-AgroParisTech, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique et de l'Environnement (INRAE), 78026 Versailles, France.
Microorganisms. 2023 Jan 12;11(1):199. doi: 10.3390/microorganisms11010199.
The application of bacterial bio-inputs is a very attractive alternative to the use of mineral fertilisers. In ploughed soils including a crop rotation pea, we observed an enrichment of bacterial communities with (.) . Inoculation experiments, cytological studies, and sequencing were used to investigate the beneficial role of in pea. is able to colonise pea plants and establish a symbiotic association that promotes plant biomass production. Sequencing of the genome revealed the existence of genes involved in secretion systems, Nod factor synthesis, and nitrogenase activity. Light and electron microscopic observations allowed us to refine the different steps involved in the establishment of the symbiotic association, including the formation of infection threads, the entry of the bacteria into the root cells, and the development of differentiated bacteroids in root nodules. These results, together with phylogenetic analysis, demonstrated that is a non-rhizobia that has the potential to develop a beneficial symbiotic association with a legume. Such a symbiotic association could be a promising alternative for the development of more sustainable agricultural practices, especially under reduced N fertilisation conditions.
细菌生物投入物的应用是使用矿物肥料的一种非常有吸引力的替代方法。在包括轮作豌豆的深耕土壤中，我们观察到细菌群落富含（.）。接种实验、细胞学研究和测序被用于研究（.）在豌豆中的有益作用。（.）能够定殖于豌豆植株并建立促进植物生物量生产的共生关系。（.）基因组测序揭示了参与分泌系统、结瘤因子合成和固氮酶活性的基因的存在。光学和电子显微镜观察使我们能够细化共生关系建立过程中涉及的不同步骤，包括感染丝的形成、细菌进入根细胞以及根瘤中分化类菌体的发育。这些结果与系统发育分析一起表明，（.）是一种非根瘤菌，有潜力与豆科植物建立有益的共生关系。这种共生关系可能是发展更可持续农业实践的一个有前途的替代方法，特别是在减少氮肥施用条件下。
