Universidade Católica de Brasília, Pós-Graduação em Ciências Genômicas e Biotecnologia, SGAN Quadra 916, Módulo B, Av. W5 Norte 70.790-160-Asa Norte, Brasília/DF, Brazil.
J Biomed Mater Res A. 2010 Jul;94(1):169-79. doi: 10.1002/jbm.a.32701.
H(2)SO(4)/H(2)O(2) treatment of titanium implants imparts nanofeatures to the surface and alters the osteoblast response. The aim of this study was to evaluate the effect of H(2)SO(4)/H(2)O(2) treatment of commercially pure Titanium (cpTi) surfaces on gene expression of human mesenchymal stem cells (hMSCs) differentiated into osteoblasts. Commercially pure grade IV titanium disks (20.0 mm x 1.0 mm) were polished or polished and subsequently treated by grit blasting or grit-blasting/acid etching with an H(2)SO(4)/H(2)O(2) solution. The surfaces were divided into three groups: smooth (S), grit-blasted (Gb), and nanostructured: grit-blasted/acid etched (Nano). Surfaces were examined by scanning electron microscopy and atomic force microscopy. HMSCs were grown on the disks. The data points analyzed were at 3, 7, 14, and 28 days. Real-time PCR was used to measure the mRNA levels of ALP, BSP, Runx2, OCN, OPN, and OSX. The housekeeping gene GAPDH was used as a control. Descriptive statistics were calculated using Microsoft Excel. T-test was performed for comparison of mRNA levels when compared with S surfaces (p < 0.05). All osteoblast-specific genes were regulated in surface-dependent patterns and most of them were upregulated on the Nano surfaces. Runx2 and OSX mRNAs were more than threefold upregulated at days 14 and 28 on Nano. Higher levels for ALP (38-fold), BSP (76-fold), and OCN (3-fold) were also observed on the Nano surfaces. A grit-blasted surface imparted with nanofeatures by H(2)SO(4)/H(2)O(2) treatment affected adherent cell bone-specific gene expression. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.
硫酸/过氧化氢处理钛植入物赋予表面纳米特征，并改变成骨细胞的反应。本研究旨在评估硫酸/过氧化氢处理商用纯钛（cpTi）表面对成骨细胞分化的人骨髓间充质干细胞（hMSCs）基因表达的影响。商用纯钛四级圆盘（20.0mm×1.0mm）经抛光或抛光后，用砂粒喷砂或砂粒喷砂/酸蚀处理，并用硫酸/过氧化氢溶液处理。表面分为三组：光滑（S）、喷砂（Gb）和纳米结构：喷砂/酸蚀（Nano）。通过扫描电子显微镜和原子力显微镜观察表面。将 HMSCs 生长在磁盘上。分析的数据点为 3、7、14 和 28 天。实时 PCR 用于测量碱性磷酸酶（ALP）、骨涎蛋白（BSP）、Runx2、骨钙素（OCN）、骨桥蛋白（OPN）和骨特异性碱性磷酸酶（OSX）的 mRNA 水平。管家基因 GAPDH 用作对照。使用 Microsoft Excel 计算描述性统计数据。当与 S 表面相比时，使用 T 检验比较 mRNA 水平（p<0.05）。所有成骨细胞特异性基因均以依赖表面的模式进行调节，其中大多数在纳米表面上调。在第 14 和 28 天，Nano 表面上的 Runx2 和 OSX mRNA 上调超过三倍。Nano 表面上还观察到更高水平的碱性磷酸酶（38 倍）、骨涎蛋白（76 倍）和骨钙素（3 倍）。硫酸/过氧化氢处理赋予砂粒喷砂表面纳米特征，影响附着细胞的骨特异性基因表达。（c）2010 Wiley Periodicals，Inc. J Biomed Mater Res，2010。
