Cooper Lyndon F, Zhou Yongsheng, Takebe Jun, Guo Juanli, Abron Armin, Holmén Anders, Ellingsen Jan Eirik
Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 404 Brauer Hall, CB 7450, Chapel Hill, NC 27511, USA.
Biomaterials. 2006 Feb;27(6):926-36. doi: 10.1016/j.biomaterials.2005.07.009. Epub 2005 Aug 19.
Increasing bone formation at endosseous titanium implants may be achieved by modification of topographically enhanced surfaces. The aim of this study was to determine the effect of fluoride ion modification of TiO2 grit-blasted, c.p. titanium implants on osteoblastic differentiation and interfacial bone formation by parallel in vitro and in vivo investigations. Human mesenchymal stem cells (Osiris Therapeutics, Inc.) were cultured on TiO2 grit-blasted c.p.titanium disks with and without fluoride ion modification. Cell adhesion, proliferation, and osteoblastic gene expression was measured by scanning electron microscopy, tritiated-thymidine uptake into insoluble DNA, and reverse transcription polymerase chain reaction detection of mRNAs encoding collagen 1, osteopontin, bone sialoprotein, osteocalcin and BMP-2. After 24 h, there were no differences in cell adhesion among the surfaces tested. Fluoride-treated surfaces supported greater proliferation and increased bone sialoprotein and BMP-2 expression. Additionally, 12 TiO2 grit-blasted and 12 fluoride ion modified implants were placed randomly into medial and distal osteotomies prepared in the tibia of 300 g Sprague Dawley rats. After 21 days, the tibiae were harvested and 100 microm ground sections were examined by backscatter scanning electron microscopy. The bone-to-implant contact formed at TiO2 grit-blasted and fluoride-treated versus TiO2 grit-blasted surfaces was 55.45% versus 34.21% (p<0.027), respectively. Fluoride ion modification of the TiO2 grit-blasted surface enhanced osteoblastic differentiation in vitro and interfacial bone formation in vivo. This parallel in vitro and in vivo investigation demonstrates that fluoride ion modification enhanced osteoblastic differentiation and interfacial bone formation. The mechanism(s) by which fluoride ion modification of c.p.titanium enhanced osteoblastic differentiation and osseointegration merit careful investigation.
通过对具有形貌增强表面的钛种植体进行改性，可实现骨内钛种植体骨形成的增加。本研究的目的是通过体外和体内平行研究，确定氟离子改性的TiO2喷砂纯钛种植体对成骨细胞分化和界面骨形成的影响。将人间充质干细胞（Osiris Therapeutics公司）培养在有或没有氟离子改性的TiO2喷砂纯钛盘上。通过扫描电子显微镜、氚标记胸腺嘧啶核苷掺入不溶性DNA以及逆转录聚合酶链反应检测编码胶原蛋白1、骨桥蛋白、骨唾液蛋白、骨钙素和骨形态发生蛋白2的mRNA来测量细胞粘附、增殖和成骨细胞基因表达。24小时后，测试的表面之间细胞粘附没有差异。氟处理的表面支持更大的增殖，并增加了骨唾液蛋白和骨形态发生蛋白2的表达。此外，将12个TiO2喷砂种植体和l2个氟离子改性种植体随机植入300g斯普拉格-道利大鼠胫骨制备的内侧和远端截骨处。21天后，收获胫骨，用背散射扫描电子显微镜检查100微米的磨片。在TiO2喷砂和氟处理表面与TiO2喷砂表面形成的骨与种植体接触分别为55.45%和34.21%（p<0.027）。TiO2喷砂表面的氟离子改性增强了体外成骨细胞分化和体内界面骨形成。这种体外和体内平行研究表明，氟离子改性增强了成骨细胞分化和界面骨形成。纯钛的氟离子改性增强成骨细胞分化和骨结合的机制值得深入研究。
