Singer Thomas, Brand Vanessa, Moehrlen Ueli, Fehrenbach Heinz, Purkabiri Kurosch, Ott Sebastian Robert, Stammberger Uz, Ochs Matthias, Hamacher Jürg
Internal Medicine V/Pulmonary Division, and Clinical and Experimental Surgery, University Hospital Homburg/Saar, University of Saarland, Germany.
Exp Lung Res. 2010 Feb;36(1):25-30. doi: 10.3109/01902140903042597.
A method of left main bronchus intubation was developed based on a wire guide-based microscopic endotrachael mouse intubation technique. The authors used a 22 G x 1 inch catheter elongated by a 38-mm silicone tube, and a wire guide with a tag to assign the length of the wire completely covered by the silicon tube. The isoflurane-anesthetized mouse was hung perpendicularly with its incisors on a thread and transorally intubated under strict vision with the wire guide tip advanced 3 mm out of the catheter. Then the catheter was advanced about 6 to 8 mm into the trachea. Afterwards the wire guide was redrawn to the level of the catheter tip (blue tag on the wire guide appeared at the upper end of catheter) to prevent injury. Then the neck was pushed into a right lateral flexion with one finger against a foam block fixed on the vertical plate, causing a straight distance between mouth and left main bronchus. This positioning allows to gently advance the catheter into the left main bronchus by another about 20 mm, using the wire guide with its tip just within the tube, to achieve there a wedge position with gentle pressure.The technique had a success rate of more than 80% in 81 mice weighing 23 to 48 g. It may be of interest for unilateral lung intervention, e.g., with injurious substances or with drugs.
基于一种基于导丝的小鼠显微气管插管技术，开发了一种左主支气管插管方法。作者使用了一根由38毫米硅胶管延长的22G×1英寸导管，以及一根带有标签的导丝，以确定被硅胶管完全覆盖的导丝长度。将异氟烷麻醉的小鼠垂直悬挂，使其门齿挂在一根线上，并在严格的视野下经口插管，将导丝尖端从导管中伸出3毫米。然后将导管推进气管约6至8毫米。之后，将导丝回拉至导管尖端水平（导丝上的蓝色标签出现在导管上端）以防止损伤。然后用一根手指将颈部推向右侧屈曲，手指抵住固定在垂直板上的泡沫块，使口腔与左主支气管之间形成一条直线距离。这种定位允许在导丝尖端刚好在管内的情况下，再将导管轻轻推进左主支气管约20毫米，以在那里通过轻轻施压实现楔形位置。该技术在81只体重为23至48克的小鼠中的成功率超过80%。它可能对单侧肺干预（例如使用有害物质或药物）有意义。
