Sasaki Kazumasu, Ito Takuya, Shiga Takuya, Uemura Kazunori, Ishikawa Tatsuya
Department of Anesthesiology, Akita Cerebrospinal and Cardiovascular Center, Research Institute for Brain and Blood Vessels, Akita, Japan.
Research Technology Support Center, Asahikawa Medical University, Hokkaido, Japan.
J Vet Med Sci. 2025 Jun 1;87(6):589-593. doi: 10.1292/jvms.25-0013. Epub 2025 Apr 10.
Endotracheal intubation plays a crucial role in airway management in mice. Transoral endotracheal intubation (TEI) offers a less invasive alternative to tracheostomy and is more ideal for chronic experiments. Despite the development of various TEI techniques in mice, the lack of accessible and well-established methods that do not require specialized equipment hinders routine research. We developed a feasible and highly successful method for TEI in C57BL/6JJcl mice using a combination of commercially available research equipment. A success rate of 97.5% was achieved within 21.2 ± 8.9 sec (mean [standard deviation]) with no observed complications in the 40 mice studied. This method simplifies TEI without specialized tools and provides practical guidance for consistent application in routine research settings.
气管插管在小鼠气道管理中起着关键作用。经口气管插管（TEI）为气管切开术提供了一种侵入性较小的替代方法，并且更适合慢性实验。尽管已经开发出多种小鼠TEI技术，但缺乏无需专门设备的易于获取且成熟的方法阻碍了常规研究。我们使用市售研究设备组合，开发了一种适用于C57BL/6JJcl小鼠的可行且非常成功的TEI方法。在所研究的40只小鼠中，在21.2±8.9秒（平均值[标准差]）内成功率达到97.5%，未观察到并发症。该方法无需专门工具即可简化TEI，并为在常规研究环境中的一致应用提供了实用指导。
