深度SAGE——具有高灵敏度、简单实验方案和样本多重分析功能的数字转录组学技术。
DeepSAGE--digital transcriptomics with high sensitivity, simple experimental protocol and multiplexing of samples.
作者信息
Nielsen Kåre L, Høgh Annabeth Laursen, Emmersen Jeppe
机构信息
Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University DK-9000 Aalborg, Denmark.
出版信息
Nucleic Acids Res. 2006;34(19):e133. doi: 10.1093/nar/gkl714. Epub 2006 Oct 5.
Digital transcriptomics with pyrophosphatase based ultra-high throughput DNA sequencing of di-tags provides high sensitivity and cost-effective gene expression profiling. Sample preparation and handling are greatly simplified compared to Serial Analysis of Gene Expression (SAGE). We compare DeepSAGE and LongSAGE data and demonstrate greater power of detection and multiplexing of samples derived from potato. The transcript analysis revealed a great abundance of up-regulated potato transcripts associated with stress in dormant potatoes compared to harvest. Importantly, many transcripts were detected that cannot be matched to known genes, but is likely to be part of the abiotic stress-response in potato.
基于焦磷酸酶的双标签超高通量DNA测序的数字转录组学提供了高灵敏度和经济高效的基因表达谱分析。与基因表达序列分析（SAGE）相比，样品制备和处理大大简化。我们比较了深度SAGE和长SAGE数据，并证明了来自马铃薯的样品具有更强的检测和多重分析能力。转录本分析显示，与收获时相比，休眠马铃薯中与胁迫相关的上调马铃薯转录本大量存在。重要的是，检测到许多与已知基因不匹配的转录本，但它们可能是马铃薯非生物胁迫反应的一部分。
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