Wei Han-Tian, Hou Dan, Ashraf Muhammad Furqan, Lu Hai-Wen, Zhuo Juan, Pei Jia-Long, Qian Qi-Xia
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'An, China.
College of Landscape Architecture, Zhejiang A&F University, Lin'An, China.
Front Plant Sci. 2022 Jan 28;12:788895. doi: 10.3389/fpls.2021.788895. eCollection 2021.
Bamboo, being an ornamental plant, has myriad aesthetic and economic significance. Particularly, cv. Viridisulcata contains an internode color phenotype in variation in green and yellow color between the sulcus and culm, respectively. This color variation is unique, but the underlying regulatory mechanism is still unknown. In this study, we used metabolomic and transcriptomic strategies to reveal the underlying mechanism of variation in internode color. A total of 81 metabolites were identified, and among those, prunin as a flavanone and rhoifolin as a flavone were discovered at a high level in the culm. We also found 424 differentially expressed genes and investigated three genes (, , and ) that might be involved in prunin or rhoifolin biosynthesis. Their validation by qRT-PCR confirmed high transcript levels in the culm. The results revealed that , , and might promote the accumulation of prunin and rhoifolin which were responsible for the variation in internode color of . Our study also provides a glimpse into phenotypic coloration and is also a valuable resource for future studies.
竹子作为一种观赏植物，具有诸多美学和经济价值。特别是，绿槽竹品种的节间存在一种颜色表型变异，即槽沟和茎秆分别呈现绿色和黄色。这种颜色变异很独特，但其潜在的调控机制仍不清楚。在本研究中，我们采用代谢组学和转录组学策略来揭示节间颜色变异的潜在机制。共鉴定出81种代谢物，其中，黄烷酮类的樱草苷和黄酮类的异鼠李素在茎秆中含量较高。我们还发现了424个差异表达基因，并研究了三个可能参与樱草苷或异鼠李素生物合成的基因（，，和）。通过qRT-PCR对它们进行验证，证实其在茎秆中的转录水平较高。结果表明，，和可能促进了樱草苷和异鼠李素的积累，这两种物质导致了绿槽竹节间颜色的变异。我们的研究也为表型色素沉着提供了一个视角，并且是未来研究的宝贵资源。
