Kamińska Marta, Okrój Wiesława, Szymański Witold, Jakubowski Witold, Komorowski Piotr, Nosal Andrzej, Szymanowski Hieronim, Gazicki-Lipman Maciej, Jerczyńska Hanna, Pawłowska Zofia, Walkowiak Bogdan
Institute of Materials Science and Engineering and the Centre of Excellence NANODIAM, Technical University of Łódź, Poland.
Acta Bioeng Biomech. 2009;11(3):19-25.
The aim of the present work was to examine the interactions of parylene C with such selected biological objects as: blood plasma proteins, platelets, endothelial cells, and bacterial biofilm produced by E. coli cells. The results obtained strongly support the thesis that parylene C is a material worth considering for biomedical use. Parylene C coating on polished medical steel significantly reduces platelet adhesion to this surface. On the other hand, in the case of the surface of machined medical steel coated with parylene C, the number of adhered platelets is significantly higher. This also means that surface texture of substrate material is very well reproduced by parylene C coating and is an important factor facilitating the platelet adhesion. Adsorption of plasma proteins at parylene C surface is very effective, and this finding confirms a notion that cell interaction with surfaces is mediated by the adsorbed proteins. In the light of the above, a high susceptibility of parylene C surface to bacterial colonization is easy to explain. The results showing reduced proliferation and changes in endothelial cell gene expression should also be seriously analysed when parylene C is considered for the use in contact with blood vessels.
本研究的目的是考察聚对二甲苯C与以下所选生物对象的相互作用：血浆蛋白、血小板、内皮细胞以及大肠杆菌细胞产生的细菌生物膜。所获得的结果有力地支持了以下论点：聚对二甲苯C是一种值得考虑用于生物医学用途的材料。在抛光医用钢上涂覆聚对二甲苯C可显著降低血小板在该表面的黏附。另一方面，在涂覆有聚对二甲苯C的加工医用钢表面，黏附的血小板数量显著更高。这也意味着聚对二甲苯C涂层能很好地再现基底材料的表面纹理，并且是促进血小板黏附的一个重要因素。血浆蛋白在聚对二甲苯C表面的吸附非常有效，这一发现证实了细胞与表面的相互作用是由吸附的蛋白质介导的这一观点。鉴于上述情况，聚对二甲苯C表面对细菌定殖的高敏感性很容易解释。当考虑将聚对二甲苯C用于与血管接触时，显示其增殖减少以及内皮细胞基因表达变化的结果也应予以认真分析。
