利用功能化金纳米粒子以活性形式将膜不可渗透的酶递送至细胞内。
Intracellular delivery of a membrane-impermeable enzyme in active form using functionalized gold nanoparticles.
机构信息
Department of Chemistry, University of Massachusetts at Amherst, Amherst, Massachusetts 01003, USA.
出版信息
J Am Chem Soc. 2010 Mar 3;132(8):2642-5. doi: 10.1021/ja907887z.
Abstract
Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity.
摘要
金纳米颗粒被一层短肽包裹，以促进细胞膜不可渗透蛋白的细胞内传递。通过显微镜和酶分析，我们证明了这些颗粒能够将功能性酶输送到多种细胞系中。重要的是，被输送的蛋白质能够从内体中逃逸。此外，这些颗粒没有明显的细胞毒性。
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