Department of Nutrition, Food and Exercise Sciences, Florida State University, Tallahassee, Florida 32306-1493, USA.
J Med Food. 2010 Apr;13(2):320-5. doi: 10.1089/jmf.2009.0059.
We have reported that soy isoflavones are capable of preventing loss of bone mineral density (BMD) in rats due to ovariectomy. The intestinal microflora is important in rendering soy isoflavones bioavailability by facilitating their conversion to equol. Hence, substances that can modulate the intestinal microflora could affect the bioavailability of isoflavones. The purpose of this study was to examine whether combination of genistin and fructooligosaccharides (FOS), a prebiotic, can enhance the effects of soy isoflavones on bone in ovariectomized (OVX) female rats. Forty-eight 90-day-old female Sprague-Dawley rats were either sham-operated (Sham; one group) or Ovx (three groups) and were placed on dietary treatment for 50 days. The Sham and one Ovx group received a control diet, and the remaining Ovx groups received genistin-rich isoflavones diet (Ovx+G) or genistin-rich isoflavones and FOS diet (Ovx+G+FOS). After 50 days, blood and bone specimens were collected for analysis. The genistin-rich isoflavones diet was able to significantly increase the whole-body, right femur, and fourth lumbar BMD by 1.6%, 1.48%, and 1.3%, respectively in comparison with the Ovx control. The combination of genistin-rich isoflavones diet and 5% FOS further increased whole-body, right femur, and fourth lumbar BMD more compared to the genistin-rich isoflavones diet. Our findings suggest that although a genistin-rich isoflavones diet can increase the BMD in rats with Ovx-induced bone loss, combination of genistin-rich isoflavones and FOS had greater effect in preventing bone loss in this rat model.
我们曾报道过，大豆异黄酮能够预防去卵巢大鼠的骨密度（BMD）丢失。肠道微生物群对于将大豆异黄酮转化为大豆苷元以提高其生物利用度很重要。因此，能够调节肠道微生物群的物质可能会影响异黄酮的生物利用度。本研究旨在探讨大豆苷元和低聚果糖（FOS）的组合（一种益生元）是否可以增强大豆异黄酮对去卵巢雌性大鼠骨骼的作用。将 48 只 90 日龄雌性 Sprague-Dawley 大鼠去卵巢（OVX；3 组）或假手术（Sham；1 组），并进行 50 天的饮食处理。Sham 和 1 个 OVX 组给予对照饮食，其余 OVX 组给予富含大豆苷元异黄酮的饮食（OVX+G）或富含大豆苷元异黄酮和 FOS 的饮食（OVX+G+FOS）。50 天后，采集血液和骨骼标本进行分析。与 OVX 对照组相比，富含大豆苷元异黄酮的饮食分别使全身、右侧股骨和第四腰椎的 BMD 增加了 1.6%、1.48%和 1.3%。富含大豆苷元异黄酮的饮食与 5% FOS 的组合进一步增加了全身、右侧股骨和第四腰椎的 BMD，与富含大豆苷元异黄酮的饮食相比，增加幅度更大。我们的研究结果表明，虽然富含大豆苷元异黄酮的饮食可以增加去卵巢大鼠的 BMD，但富含大豆苷元异黄酮和 FOS 的组合在该大鼠模型中对预防骨质流失具有更大的效果。
