Kim Dae Won, Yoo Ki-Yeon, Lee Yoon-Bok, Lee Keun-Ha, Sohn Heon-Soo, Lee Sung-Joon, Cho Kyung-Hwan, Shin Yong Kook, Hwang In Koo, Won Moo-Ho, Kim Dong-Woo
Research Center, Natural F&P Corp., Hallym University, Chuncheon, Republic of Korea.
J Med Food. 2009 Jun;12(3):536-41. doi: 10.1089/jmf.2008.1027.
We evaluated the protective effects of soy isoflavones (SIF) against osteoporosis in middle-aged ovariectomized (OVX) mice. SIF (30 mg/kg or 60 mg/kg) or 17beta-estradiol (E(2)) was administered to OVX mice for 4 months after bilateral ovariectomy. We observed the biochemical markers of bone turnover, e.g., alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), in serum. We also observed the bone mineral density (BMD) in femurs and lumbar vertebrae. In addition, we examined trabecular bone and interstitial cells in the femur using hematoxylin and eosin staining. The decrease in ALP levels and the increase in TRAP levels normally resulting from ovariectomy were suppressed by administration of 60 mg/kg SIF or E(2). Administration of 60 mg/kg SIF or E(2) also maintained the BMD, trabecular bone, and interstitial cells in OVX mice compared to those in pre-OVX mice. These results suggest that 60 mg/kg SIF effectively mitigates ovariectomy-induced osteoporosis in middle-aged mice.
我们评估了大豆异黄酮（SIF）对中年去卵巢（OVX）小鼠骨质疏松症的保护作用。在双侧卵巢切除术后，将SIF（30毫克/千克或60毫克/千克）或17β-雌二醇（E₂）给予OVX小鼠，持续4个月。我们观察了血清中骨转换的生化标志物，如碱性磷酸酶（ALP）和抗酒石酸酸性磷酸酶（TRAP）。我们还观察了股骨和腰椎的骨矿物质密度（BMD）。此外，我们使用苏木精和伊红染色检查了股骨中的小梁骨和间质细胞。给予60毫克/千克SIF或E₂可抑制通常由卵巢切除术导致的ALP水平降低和TRAP水平升高。与去卵巢前的小鼠相比，给予60毫克/千克SIF或E₂还能维持OVX小鼠的骨矿物质密度、小梁骨和间质细胞。这些结果表明，60毫克/千克SIF可有效减轻中年小鼠卵巢切除术后引起的骨质疏松症。
