Notermans S, Dufrenne J, Schothorst M
Jpn J Med Sci Biol. 1978 Feb;31(1):81-5. doi: 10.7883/yoken1952.31.81.
The enzyme linked immunosorbent assay using the so-called "double-sandwich technique" has been applied to determine botulinum toxin type A. By this assay, 50-100 mouse ip LD50 of toxin type A can be detected. No cross-reaction occurs with botulinum toxins of other types tested. In all probability this is due to the high specificity of the antiserum prepared against the toxic component of type A toxin.
采用所谓“双夹心技术”的酶联免疫吸附测定法已用于检测A型肉毒杆菌毒素。通过该测定法，可检测出50 - 100个小鼠腹腔注射半数致死量的A型毒素。与所检测的其他类型肉毒杆菌毒素未发生交叉反应。很可能这是由于针对A型毒素毒性成分制备的抗血清具有高度特异性。
