肉毒杆菌毒素免疫反应的动力学研究
Kinetics study of immunological response to Clostridium botulinum toxin.
作者信息
Dezfulian M, Bitar R A, Bartlett J G
出版信息
J Clin Microbiol. 1987 Jul;25(7):1336-7. doi: 10.1128/jcm.25.7.1336-1337.1987.
Abstract
A double-antibody enzyme-linked immunosorbent assay (ELISA) for detection of humoral antibody to type A botulinal toxin was developed. This assay was used to study the kinetics of antibody response of a volunteer to botulinal toxoid. The circulating type A antitoxin was first detected by the ELISA 2 weeks after the first booster injection of the toxoid. The antibody titer stayed level until the second booster at 12 weeks. The titer then continued to rise throughout the remaining study period. The neutralizing antibody to type A toxin was detected by mouse assay 15 weeks after detection of antitoxin by the ELISA.
摘要
开发了一种用于检测A型肉毒杆菌毒素体液抗体的双抗体酶联免疫吸附测定（ELISA）。该测定用于研究一名志愿者对肉毒杆菌类毒素抗体反应的动力学。在首次注射类毒素加强针后2周，ELISA首次检测到循环中的A型抗毒素。抗体滴度在12周第二次加强针之前保持稳定。然后在剩余的研究期间滴度持续上升。在ELISA检测到抗毒素15周后，通过小鼠试验检测到对A型毒素的中和抗体。
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