Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
BMB Rep. 2010 Jan;43(1):46-51. doi: 10.5483/bmbrep.2010.43.1.046.
Thermal burn injury induces inflammatory cell infiltrates in the dermis and thickening of the epidermis. Following a burn injury, various mediators, including reactive oxygen species (ROS), are produced in macrophages and neutrophils, exposing all tissues to oxidative injury. The anti-oxidant activities of flavonoids have been widely exploited to scavenge ROS. In this study, we observed that several flavonoids-kaempferol, quercetin, fisetin, and chrysin-inhibit LPS-induced IL-8 promoter activation in RAW 264.7 cells. In contrast with quercetin and fisetin, pretreatment of kaempferol and chrysin did not decrease cell viability. Inflammatory cell infiltrates in the dermis and thickening of the epidermis induced by burn injuries in mice was relieved by kaempferol treatment. However, the injury was worsened by fisetin, quercetin, and chrysin. Expression of TNF-a induced by burn injuries was decreased by kaempferol. These findings suggest the potential use of kaempferol as a therapeutic in thermal burn-induced skin injuries. [BMB reports 2010; 43(1): 46-51].
热烧伤会引起真皮中的炎症细胞浸润和表皮增厚。烧伤后，巨噬细胞和中性粒细胞会产生各种介质，包括活性氧（ROS），使所有组织都暴露于氧化损伤之下。类黄酮的抗氧化活性已被广泛用于清除 ROS。在这项研究中，我们观察到几种类黄酮——山奈酚、槲皮素、非瑟酮和白杨素——抑制 LPS 诱导的 RAW 264.7 细胞中 IL-8 启动子的激活。与槲皮素和非瑟酮不同，山奈酚和白杨素预处理不会降低细胞活力。山奈酚处理可减轻烧伤引起的小鼠真皮中的炎症细胞浸润和表皮增厚。然而，非瑟酮、槲皮素和白杨素则会使损伤加重。烧伤引起的 TNF-a 表达减少。这些发现表明，山奈酚可能具有治疗热烧伤引起的皮肤损伤的潜力。[BMB 报告 2010；43(1): 46-51]。
