Department of Oral and Maxillofacial Surgery, Hallym University, Chuncheon 200-702, Korea.
BMB Rep. 2010 Jan;43(1):52-6. doi: 10.5483/bmbrep.2010.43.1.052.
Silk fibroin, produced by the silkworm Bombyx mori, has been widely studied as a scaffold in tissue engineering. Although it has been shown to be slowly biodegradable, cellular responses to degraded silk fibroin fragments are largely unknown. In this study, silk fibroin was added to MG-63 cell cultures, and changes in gene expression in the MG-63 cells were screened by DNA microarray analysis. Genes showing a significant (2-fold) change were selected and their expression changes confirmed by quantitative RT-PCR and western blotting. DNA microarray results showed that alkaline phosphatase (ALP), collagen type-I alpha-1, fibronectin, and transforming growth factor-beta1 expressions significantly increased. The effect of degraded silk fibroin on osteoblastogenic gene expression was confirmed by observing up-regulation of ALP activity in MG-63 cells. The finding that small fragments of silk fibroin are able to increase the expression of osteoblastogenic genes suggests that controlled degradation of silk fibroin might accelerate new bone formation. [BMB reports 2010; 43(1): 52-56].
丝素蛋白由家蚕（Bombyx mori）产生，已被广泛研究作为组织工程中的支架。尽管已经表明它是缓慢可生物降解的，但对于降解的丝素蛋白片段的细胞反应在很大程度上是未知的。在这项研究中，丝素蛋白被添加到 MG-63 细胞培养物中，并且通过 DNA 微阵列分析筛选 MG-63 细胞中的基因表达变化。选择显示出显著（2 倍）变化的基因，并通过定量 RT-PCR 和 Western blot 确认其表达变化。DNA 微阵列结果表明碱性磷酸酶（ALP）、I 型胶原α-1、纤连蛋白和转化生长因子-β1 的表达显著增加。通过观察 MG-63 细胞中 ALP 活性的上调，证实了降解丝素蛋白对成骨基因表达的影响。发现丝素蛋白的小片段能够增加成骨基因的表达，这表明丝素蛋白的可控降解可能会加速新骨形成。[BMB 报告 2010；43(1): 52-56]。
