Zhou Liang, Pan Meng, Zhang Zhenghua, Diao Zijie, Peng Xiaochun
Department of Materials and Engineering, School of Forestry and Landscape Architecture, Anhui Agriculture University, Hefei, China.
Department of Thoracic Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
Front Bioeng Biotechnol. 2021 Feb 23;9:639835. doi: 10.3389/fbioe.2021.639835. eCollection 2021.
Titanium (Ti) alloys have been applied to biomedical implants for a long time. Although Ti alloys are biocompatible, efforts have been continuously made to improve their bone conductivity and osteogenesis for enhancing their performance. Silk fibroin (SF) is a natural biomaterial with excellent biomedical and mechanical properties, and hydroxyapatite (HAP) nanocomposites derived from SF are promising for producing "artificial bone" owing to their biomedical applicability and strong mechanical functions. Therefore, we built an SF coating on the surface of Ti-6Al-4V alloy, and then the incubated SF-coated Ti alloy were immersed in simulated body fluid to induce mineral deposition of HAP on the alloys. The results from Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) analysis, and Attenuated Total Reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR) confirmed the deposition of a mineral layer on the SF film surface. The proliferation, adhesion, and differentiation of MG-63 were tested, along with the BMP-2, COX-2, and OPG expression and protein content in the MG-63. Both Ti + SF and Ti + SF + HAP groups exhibited significantly better performance than a control Ti group with regard to the cell adhesion, cell proliferation, and protein expression. Furthermore, the hybrid layer comprising HAP and SF delivered more significant improvement of the osseointegration than the SF alone. It is hoped that the proposed methods can be used for constructing modified surfaces on Ti alloys, as they endowed the implants with good osteogenic potential.
钛（Ti）合金长期以来一直应用于生物医学植入物。尽管钛合金具有生物相容性，但人们一直在不断努力提高其骨传导性和成骨能力以提升其性能。丝素蛋白（SF）是一种具有优异生物医学和机械性能的天然生物材料，由丝素蛋白衍生的羟基磷灰石（HAP）纳米复合材料因其生物医学适用性和强大的机械功能而有望用于制造“人造骨”。因此，我们在Ti-6Al-4V合金表面构建了丝素蛋白涂层，然后将经过孵育的涂有丝素蛋白的钛合金浸入模拟体液中，以诱导合金表面形成羟基磷灰石矿物沉积。扫描电子显微镜（SEM）、X射线衍射（XRD）分析和衰减全反射傅里叶变换红外光谱（ATR-FTIR）的结果证实了丝素蛋白膜表面有矿物层沉积。测试了MG-63细胞的增殖、黏附及分化情况，以及MG-63细胞中骨形态发生蛋白-2（BMP-2）、环氧化酶-2（COX-2）和骨保护素（OPG）的表达及蛋白含量。在细胞黏附、细胞增殖和蛋白表达方面，Ti + SF组和Ti + SF + HAP组均表现出明显优于对照Ti组的性能。此外，由羟基磷灰石和丝素蛋白组成的混合层在骨整合方面比单独的丝素蛋白有更显著的改善。希望所提出的方法可用于在钛合金表面构建改性表面，因为它们赋予植入物良好的成骨潜力。
