Department of Parasitology, Institute of Tropical Medicine, Nationalestraat 155, Antwerp, Belgium.
Am J Trop Med Hyg. 2010 Feb;82(2):223-7. doi: 10.4269/ajtmh.2010.09-0458.
Fourteen published and three newly identified polymorphic microsatellites were used to genotype 69 Plasmodium vivax samples obtained from 39 patients detected over a period of two years who lived in a rural community of central Vietnam. All samples were polyclonal with an average expected heterozygosity of 0.86. Among the 39 patients, 16 experienced 1-5 recurrent episodes of P. vivax malaria, most of them (83%) with a different genotype profile compared with previous infections. The minimal set of microsatellites required for differentiating the genotype profiles of the recurrent infections compared with the full set of 17 microsatellites was explored. A combination of five markers was sufficient to identify all recurrent infections with an unrelated or different genotype profile compared with all previous episodes.
14 个已发表和 3 个新鉴定的多态微卫星用于对来自越南中部一个农村社区的 39 名在两年期间检测到的患有疟疾的患者的 69 个间日疟原虫样本进行基因分型。所有样本均为多克隆，平均预期杂合度为 0.86。在 39 名患者中，有 16 人经历了 1-5 次间日疟原虫的复发性发作，其中大多数（83%）与以前的感染相比具有不同的基因型特征。探索了区分复发性感染与全部 17 个微卫星的基因型特征所需的最小微卫星集。与所有先前的发作相比，五标记组合足以识别所有具有无关或不同基因型特征的复发性感染。
