Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Republic of Korea; Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Republic of Korea.
Department of Molecular Parasitology and Tropical Diseases, School of Medicine and Research Center of International Tropical Medicine, College of Medicine, Taipei Medical University, 250 Wu-Xing Street, Taipei, Taiwan.
Parasitol Int. 2021 Aug;83:102374. doi: 10.1016/j.parint.2021.102374. Epub 2021 May 3.
Vietnam achieved outstanding success against malaria in the last few decades. The mortality and morbidity of malaria in Vietnam have decreased remarkably in recent years, but malaria is still a major public health concern in the country, particularly in the Central Highlands region. In this study, molecular analyses of malaria parasites in the Central Highlands were performed to understand the population structure and genetic diversity of the parasites circulating in the region. Plasmodium falciparum (68.7%) and P. vivax (27.4%) along with mixed infections with P. falciparum/P. vivax (3.9%) were detected in 230 blood samples from patients with malaria. Allele-specific nested-polymerase chain reaction (PCR) or PCR-restriction fragment length polymorphism (PCR-RFLP) analyses of pfmsp-1, pfama-1, pvcsp, and pvmsp-1 revealed complex genetic makeup in P. falciparum and P. vivax populations of Vietnam. Substantial multiplicity of infection (MOI) was also identified, suggesting significant genetic diversity and polymorphism of P. falciparum and P. vivax populations in the Central Highlands of Vietnam. These results provide fundamental insight into the current patterns of dispersion and genetic nature of malaria parasites as well as for the development of malaria elimination strategies in the endemic region.
越南在过去几十年中在抗击疟疾方面取得了卓越的成就。近年来，越南疟疾的死亡率和发病率显著下降，但疟疾仍然是该国的一个主要公共卫生关注点，特别是在中高原地区。在这项研究中，对中高原地区的疟原虫进行了分子分析，以了解该地区流行的寄生虫的种群结构和遗传多样性。从 230 名疟疾患者的血液样本中检测到了恶性疟原虫（68.7%）和间日疟原虫（27.4%）以及恶性疟原虫/间日疟原虫混合感染（3.9%）。pfmsp-1、pfama-1、pvcsp 和 pvmsp-1 的等位基因特异性巢式聚合酶链反应（PCR）或 PCR-限制性片段长度多态性（PCR-RFLP）分析显示，越南恶性疟原虫和间日疟原虫种群的遗传结构复杂。还发现了大量的感染多重性（MOI），这表明越南中高原地区的恶性疟原虫和间日疟原虫种群具有显著的遗传多样性和多态性。这些结果为了解疟疾寄生虫的当前分布模式和遗传特性提供了基础，并为在流行地区制定消除疟疾策略提供了依据。
