Sellami M H, Ben Ahmed A, Kaabi H, Jridi A, Dridi A, Hmida S
Department of Immunohaematology, National Blood Transfusion Center of Tunis, Tunis, Tunisia.
Tissue Antigens. 2010 Jun;75(6):720-3. doi: 10.1111/j.1399-0039.2010.01444.x. Epub 2010 Jan 28.
Minor histocompatibility antigens (MiHAgs), such as HA-1 and HA-2, are the main targets of immune responses after allogeneic stem cell transplantation (SCT). HA-1 and HA-2 are two hematopoietic system-restricted antigens encoded, respectively, by HMHA1 and MYO1G genes. In order to estimate their frequencies in Tunisians, we performed a molecular-based allele analysis for 160 healthy and unrelated subjects. Genomic DNAs were extracted mainly by the salting out method. Single nucleotide polymorphism (SNP) genotyping assays for selected sites at HMHA1 gene (rs3764653 and rs1801284) and at MYO1G gene (rs61739531) were performed with a sequence specific primers-polymerase chain reaction (SSP-PCR) method. Statistical analysis of our results showed that the HA-2 antigen is more frequent than the HA-1 antigen in the Tunisian population because their frequencies were 97% and 57%, respectively. Allele analysis for HMHA1 gene showed that the R variant (500T-504G) was predominant in our population (64%). For the MYO1G gene, the C allele was predominant (84%). All loci were in Hardy-Weinberg equilibrium (minimum P value = 0.06). Our frequencies were close to those reported in African and Caucasian groups.
微小组织相容性抗原（MiHAgs），如HA - 1和HA - 2，是异基因干细胞移植（SCT）后免疫反应的主要靶点。HA - 1和HA - 2是分别由HMHA1和MYO1G基因编码的两种造血系统限制性抗原。为了评估它们在突尼斯人群中的频率，我们对160名健康且无亲缘关系的受试者进行了基于分子的等位基因分析。基因组DNA主要通过盐析法提取。采用序列特异性引物 - 聚合酶链反应（SSP - PCR）方法，对HMHA1基因（rs3764653和rs1801284）以及MYO1G基因（rs61739531）的选定位点进行单核苷酸多态性（SNP）基因分型检测。我们的结果统计分析表明，在突尼斯人群中，HA - 2抗原比HA - 1抗原更常见，因为它们的频率分别为97%和57%。HMHA1基因的等位基因分析显示，R变体（500T - 504G）在我们的人群中占主导地位（64%）。对于MYO1G基因，C等位基因占主导地位（84%）。所有位点均处于哈迪 - 温伯格平衡（最小P值 = 0.06）。我们得到的频率与非洲和高加索人群组报道的频率相近。
