人星状病毒 6 型全基因组序列

Complete genome sequence of human astrovirus genotype 6.

机构信息

Dr, Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing 100730, PR China.

出版信息

Virol J. 2010 Feb 8;7:29. doi: 10.1186/1743-422X-7-29.

DOI:10.1186/1743-422X-7-29

PMID:20137100

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2829535/

Abstract

BACKGROUND

Human astroviruses (HAstVs) are one of the important causes of acute gastroenteritis in children. Currently, eight HAstV genotypes have been identified and all but two (HAstV-6 and HAstV-7) have been fully sequenced. We here sequenced and analyzed the complete genome of a HAstV-6 strain (192-BJ07), which was identified in Beijing, China.

RESULTS

The genome of 192-BJ07 consists of 6745 nucleotides. The 192-BJ07 strain displays a 77.2-78.0% nucleotide sequence identity with other HAstV genotypes and exhibits amino acid sequence identities of 86.5-87.4%, 94.2-95.1%, and 65.5-74.8% in the ORF1a, ORF1b, and ORF2 regions, respectively. Homological analysis of ORF2 shows that 192-BJ07 is 96.3% identical to the documented HAstV-6 strain. Further, phylogenetic analysis indicates that different genomic regions are likely undergoing different evolutionary and selective pressures. No recombination event was observed in HAstV-6 in this study.

CONCLUSION

The completely sequenced and characterized genome of HAstV-6 (192-BJ07) provides further insight into the genetics of astroviruses and aids in the surveillance and control of HAstV gastroenteritis.

摘要

背景

人类星状病毒（HAstV）是导致儿童急性肠胃炎的重要原因之一。目前已鉴定出 8 种 HAstV 基因型，除两种（HAstV-6 和 HAstV-7）外，其余均已完成全序列测序。本研究对在中国北京鉴定出的 HAstV-6 株（192-BJ07）进行了全基因组测序和分析。

结果

192-BJ07 基因组由 6745 个核苷酸组成。该 192-BJ07 株与其他 HAstV 基因型的核苷酸序列相似度为 77.2-78.0%，在 ORF1a、ORF1b 和 ORF2 区的氨基酸序列相似度分别为 86.5-87.4%、94.2-95.1%和 65.5-74.8%。ORF2 的同源性分析表明，192-BJ07 与已报道的 HAstV-6 株完全一致。进一步的系统进化分析表明，不同基因组区域可能经历了不同的进化和选择压力。本研究中未观察到 HAstV-6 的重组事件。