Department of Microbiology, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Virol J. 2010 Sep 10;7:221. doi: 10.1186/1743-422X-7-221.
Human astroviruses (HAstVs) are among the major causes of gastroenteritis in South Korea. In this study, the partial regions of the open reading frame (ORF) 1a and ORF2 genes of HAstVs from gastroenteritis patients in nine hospitals were sequenced, and the molecular characterization of the viruses was revealed. 89 partial nucleotide sequences of ORF1a and 88 partial nucleotide sequences of ORF2 were amplified from 120 stool specimens. Phylogenetic analysis showed that most of the nucleotide sequences of ORF1a and ORF2 were grouped with HAstV type 1 but had evolutionary genetic distance compared with the reference sequences, such as the HAstV-1 prototype, Dresden strain, and Oxford strain. According to the phylogenetic analysis, some nucleotide sequences including SE0506041, SE0506043, and SE0506058, showed the discrepancy of the genotypes, but there was no proof of recombination among the HAstV types. In conclusion, this study showed that the dominant HAstV isolated from the Seoul metropolitan area in 2004-2005 was HAstV type 1, and that Korean HAstV-1 had the genetic distance in evolution compared with the reference sequences of HAstVs. Lots of nucleotide sequences of the ORF1a and ORF2 genes of HAstV will be useful for studying for the control and prevention of HAstV gastroenteritis in South Korea.
人类星状病毒（HAstV）是导致韩国肠胃炎的主要病原体之一。本研究对来自韩国九家医院的肠胃炎患者粪便标本中 HAstV 的开放阅读框（ORF）1a 和 ORF2 基因的部分区域进行了测序，并对病毒的分子特征进行了分析。从 120 份粪便标本中扩增出了 89 个 ORF1a 部分核苷酸序列和 88 个 ORF2 部分核苷酸序列。系统进化分析显示，大多数 ORF1a 和 ORF2 的核苷酸序列与 HAstV 型 1 聚为一簇，但与参考序列（如 HAstV-1 原型株、Dresden 株和 Oxford 株）相比，存在进化遗传距离。根据系统进化分析，一些核苷酸序列（包括 SE0506041、SE0506043 和 SE0506058）的基因型存在差异，但未发现 HAstV 型之间存在重组。总之，本研究表明 2004-2005 年首尔地区分离的优势 HAstV 为 HAstV 型 1，与 HAstV 的参考序列相比，韩国 HAstV-1 存在进化遗传距离。HAstV ORF1a 和 ORF2 基因的大量核苷酸序列将有助于研究韩国 HAstV 肠胃炎的控制和预防。
