Zhang Xue-yan, Zheng Bi-qiang, Han Bao-hui, Huang Jin-su, Geng Qin, Xu Hui-li, Zhou Jin, Dong Qiang-gang
Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China.
Zhonghua Zhong Liu Za Zhi. 2009 Nov;31(11):836-40.
To detect the cancer stem cells and to evaluate their prognostic implication in patients with lung adenocarcinoma.
Three phenotypic markers of cancer stem cells (SP-C, CCSP and OCT4) in lung adenocarcinoma were detected by immunofluorecence staining. The correlation among the clinicopathological parameters and phenotypes of cancer stem cells as well as survival were analyzed by Cox proportional hazard method.
Of the 57 cases, cancer stem cells were detected in 52, including OCT4(+) bronchioloalveolar stem cell (BASC) phenotype (SP-C(+) CCSP(+) OCT4(+)) in 40 cases and OCT4(-) BASC phenotype (SP-C(+) CCSP(+) OCT4(-)) in 12 cases. Statistical analysis revealed that the phenotype of cancer stem cells was related with the cellular differentiation, i.e. the OCT4(+) BASC phenotype occurred more frequently in the well-differentiated tumors, while the OCT4(-) BASC phenotype usually presented in most of the poorly-differentiated ones. Cox analysis showed that the OCT4(+) BASC phenotype was one of prognostic factors.
The lung adenocarcinoma stem cells have phenotypic features of bronchioalveolar stem cells (SP-C(+) CCSP(+)). The expression of self-renewal regulatory gene OCT4 in these cells indicates an aggressive nature and unfavorable prognosis.
检测肺腺癌患者的癌症干细胞并评估其预后意义。
采用免疫荧光染色法检测肺腺癌中癌症干细胞的三种表型标志物（SP-C、CCSP和OCT4）。采用Cox比例风险法分析癌症干细胞的临床病理参数、表型与生存之间的相关性。
57例患者中，52例检测到癌症干细胞，其中40例为OCT4(+)细支气管肺泡干细胞（BASC）表型（SP-C(+) CCSP(+) OCT4(+)），12例为OCT4(-) BASC表型（SP-C(+) CCSP(+) OCT4(-)）。统计分析显示，癌症干细胞的表型与细胞分化相关，即OCT4(+) BASC表型在高分化肿瘤中更常见，而OCT4(-) BASC表型通常出现在大多数低分化肿瘤中。Cox分析表明，OCT4(+) BASC表型是预后因素之一。
肺腺癌干细胞具有细支气管肺泡干细胞的表型特征（SP-C(+) CCSP(+)）。这些细胞中自我更新调节基因OCT4的表达表明其具有侵袭性和不良预后。
