Liu Si-yu, Wu Fan, Tao Yi-ming, Yang Lian-yue
Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhonghua Wai Ke Za Zhi. 2009 Nov 15;47(22):1732-5.
To investigate the expression of Abi1 in hepatocellular carcinoma (HCC) and the correlation between its expression level and clinical pathological characteristics as well as prognosis of HCC.
Abi1 expression was determined at both mRNA and protein levels in 40 HCC tissues and their corresponding para carcinomatous liver tissues by RT-PCR and immunohistochemistry. The correlations between Abi1 expression levels and pathological characteristics as well as prognosis were also analyzed.
The expression level of Abi1 mRNA in HCC tissues were significantly higher than those in corresponding para carcinomatous liver tissue (P < 0.05), and the expression level of Abi1 mRNA in nodular HCC tissues were also significantly higher than those in solitary large HCC tissues. Immunohistochemistry results showed that Abi1 protein located in cytoplasm of HCC cells and the expression level of Abi1 protein were significant positive correlated with the number of HCC, capsular formation, venous invasion and Edmondson-Steiner grade (P < 0.05). Combined with follow-up data, the results also showed that HCC patients with high Abi1 protein expression had a higher risk of invasion/metastasis and a shorter survival than those with low Abi1 protein expression (P < 0.05).
Expression level of Abi1 is up-regulated in HCC tissues compared with corresponding para carcinomatous liver tissue and the expression level of Abi1 is significantly correlated with the number of tumor, capsular formation, venous invasion, Edmondson-Steiner grade and prognosis of HCC.
探讨Abi1在肝细胞癌（HCC）中的表达及其表达水平与HCC临床病理特征和预后的相关性。
采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法，检测40例HCC组织及其相应癌旁肝组织中Abi1的mRNA和蛋白表达水平，并分析Abi1表达水平与病理特征及预后的相关性。
HCC组织中Abi1 mRNA表达水平显著高于相应癌旁肝组织（P<0.05），结节型HCC组织中Abi1 mRNA表达水平也显著高于孤立性大HCC组织。免疫组织化学结果显示，Abi1蛋白定位于HCC细胞胞质中，其蛋白表达水平与HCC数量、包膜形成、静脉侵犯及Edmondson-Steiner分级呈显著正相关（P<0.05）。结合随访资料，结果还显示，Abi1蛋白高表达的HCC患者侵袭/转移风险较高，生存期较Abi1蛋白低表达患者短（P<0.05）。
与相应癌旁肝组织相比，HCC组织中Abi1表达水平上调，且Abi1表达水平与肿瘤数量、包膜形成、静脉侵犯、Edmondson-Steiner分级及HCC预后显著相关。
