Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
Parasite Immunol. 2011 Jun;33(6):322-9. doi: 10.1111/j.1365-3024.2011.01283.x.
Neurocysticercosis (NC), caused by Taenia solium metacestode, infects the central nervous system and is a devastating parasitic infection. Diagnosis is based on symptoms, imaging, serology and epidemiology. Current markers present variable sensitivity and specificity, frequent cross-reactions and are not able to discriminate NC clinical forms. The aim of this study was to select mimotopes of T. solium metacestode antigens that may be used in NC immunodiagnosis, specifically to discriminate between active and inactive forms. A random peptide phage display library was screened against IgY from chickens immunized with total saline extract from T. solium metacestodes and validated against 110 serum samples, classified into active NC (18), inactive NC (22), cross-reactive parasitic diseases (40) and healthy controls (30). We have successfully selected seven peptides with significant immunoreactivity to IgG of NC patients, with sensitivity ranging from 95.5% to 100% to detect the inactive form and specificity varied from 85.7% to 94.3%. One phage-displayed peptide (Cc48) can be directly used as biomarker to distinguish inactive from active forms with an accuracy of 95.7%, and this novel mimotope may also be used as an auxiliary tool to neuroimaging tests and treatment follow-up.
神经囊尾蚴病（NC）是由猪带绦虫囊尾蚴引起的中枢神经系统寄生虫感染，具有破坏性。诊断基于症状、影像学、血清学和流行病学。目前的标志物存在敏感性和特异性差异，频繁出现交叉反应，无法区分 NC 的临床形式。本研究旨在筛选猪带绦虫囊尾蚴抗原的模拟表位，用于 NC 免疫诊断，特别是区分活动期和非活动期。针对用猪带绦虫囊尾蚴总盐水提取物免疫的鸡 IgY 进行随机肽噬菌体展示文库筛选，并对 110 份血清样本进行验证，这些样本分为活动期 NC（18 例）、非活动期 NC（22 例）、交叉反应寄生虫病（40 例）和健康对照组（30 例）。我们成功地选择了七个对 NC 患者 IgG 具有显著免疫反应性的肽，其对检测非活动期的敏感性为 95.5%至 100%，特异性为 85.7%至 94.3%。一个噬菌体展示的肽（Cc48）可以直接用作生物标志物，以 95.7%的准确率区分非活动期和活动期，这种新型模拟表位也可以用作神经影像学检查和治疗随访的辅助工具。
