Ribeiro Vanessa da Silva, Gonzaga Henrique Tomaz, Nunes Daniela da Silva, Goulart Luiz Ricardo, Costa-Cruz Julia Maria
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, 38400-902, Brazil.
Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, 38400-902, Brazil.
Parasitol Res. 2019 May;118(5):1657-1660. doi: 10.1007/s00436-019-06288-9. Epub 2019 Mar 15.
Herein, we evaluate a mimotope-based synthetic peptidenamed NC1 to diagnose neurocysticercosis (NC), a neglected parasitic disease and a major cause of epilepsy worldwide. NC1 synthetic peptide was evaluated to diagnose NC, and total saline extract from Taenia solium metacestodes (SE) was used as control. Serum samples from patients with NC (n = 40), other parasitic diseases (n = 43), and healthy individuals (n = 40) were tested. Diagnostic parameters such as sensitivity (Se), specificity (Sp), likelihood ratio (LR), and area under curve (AUC) were calculated using receiver operating characteristic (ROC) curves. The sequence from T. solium phosphoenolpyruvate carboxykinase (PEPCK) was used for epitope prediction, resulting in one high-scoring patch centered at residue L247. NC1 synthetic peptide reached high diagnostic performance (Se 97.5% and Sp 97.5%, LR+ 39 and AUC 0.997). Data from diagnostic parameters and in silico analyses proved the usefulness of NC1 synthetic peptide as a diagnostic marker for human NC.
在此，我们评估了一种基于模拟表位的合成肽NC1用于诊断神经囊尾蚴病（NC），这是一种被忽视的寄生虫病，也是全球癫痫的主要病因。对NC1合成肽进行了诊断NC的评估，并使用来自猪带绦虫囊尾蚴的总盐水提取物（SE）作为对照。检测了NC患者（n = 40）、其他寄生虫病患者（n = 43）和健康个体（n = 40）的血清样本。使用受试者工作特征（ROC）曲线计算诊断参数，如敏感性（Se）、特异性（Sp）、似然比（LR）和曲线下面积（AUC）。使用猪带绦虫磷酸烯醇丙酮酸羧激酶（PEPCK）的序列进行表位预测，结果得到一个以L247残基为中心的高分区域。NC1合成肽具有较高的诊断性能（Se 97.5%，Sp 97.5%，LR+ 39，AUC 0.997）。诊断参数数据和计算机模拟分析证明了NC1合成肽作为人类NC诊断标志物的有效性。
