Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; College of Forest Science, Kim Il Sung University, Pyongyang 999093, Democratic People's Republic of Korea.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Jan 1;1188:123077. doi: 10.1016/j.jchromb.2021.123077. Epub 2021 Dec 3.
It is of great theoretical interest and industrial significance to improve the extraction efficiency of baicalein and wogonin from Scutellaria baicalensis roots because of their high pharmacological activities. The present study was aimed to establish the optimized ultrasound-assisted enzymatic pretreatment (UAEP) process by which ultrasound irradiation and the exogenous enzyme were simultaneously applied to efficiently transform baicalin and wogonoside into baicalein and wogonin, enhancing their extraction efficiency. Single-factor experiment and Box-Behnken design were used to optimize the main UAEP conditions to maximize the total extraction yield of baicalein and wogonin. The optimized UAEP conditions were cellulase concentration of 1.1%, pH of 5.5, UAEP temperature of 56.5 °C, UAEP time of 39.4 min, and ultrasonic power of 200 W with the total extraction yield of 82.51 ± 0.85 mg/g DW. The comparison of the established technique with the reference method based on the enzymatic pretreatment revealed that the productive efficiency was significantly improved with the transformation rates nearly doubled. These results suggest that the optimized UAEP process has the potential to be applied for the green, simple, and efficient extraction of baicalein and wogonin in the pharmaceutical and food industry.
由于黄芩苷和黄芩素具有很高的药理活性，因此提高黄芩根中黄芩苷和黄芩素的提取效率具有重要的理论意义和工业意义。本研究旨在建立优化的超声辅助酶预处理（UAEP）工艺，通过同时应用超声辐射和外源酶，将黄芩苷和黄芩苷元高效转化为黄芩素和汉黄芩素，提高其提取效率。采用单因素实验和 Box-Behnken 设计优化主要 UAEP 条件，以最大限度地提高黄芩素和汉黄芩素的总提取收率。优化的 UAEP 条件为纤维素酶浓度 1.1%、pH 值 5.5、UAEP 温度 56.5°C、UAEP 时间 39.4min 和超声功率 200W，总提取收率为 82.51±0.85mg/g DW。与基于酶预处理的参考方法相比，所建立的技术表明生产效率显著提高，转化率几乎翻了一番。这些结果表明，优化的 UAEP 工艺具有在医药和食品工业中绿色、简单、高效提取黄芩素和汉黄芩素的潜力。
