Reproductive and Developmental Biology, Maternal and Child Health Science Laboratories, Centre for Oncology and Molecular Medicine, Ninewells Hospital, University of Dundee, Dundee DD1 9SY, UK.
Hum Reprod. 2010 Apr;25(4):824-38. doi: 10.1093/humrep/dep465. Epub 2010 Feb 6.
This article reports the results of the most recent in a series of EHSRE workshops designed to synthesize the current state of the field in Andrology and provide recommendations for future work (for details see Appendix). Its focus is on methods for detecting sperm DNA damage and potential application of new knowledge about sperm chromatin organization, vulnerability and repair to improve the diagnosis and treatment of clinical infertility associated with that damage. Equally important is the use and reliability of these tests to identify the extent to which environmental contaminants or pharmaceutical agents may contribute to the incidence of sperm DNA damage and male fertility problems. A working group (for workshop details, see Appendix) under the auspices of ESHRE met in May 2009 to assess the current knowledgebase and suggest future basic and clinical research directions. This document presents a synthesis of the working group's understanding of the recent literature and collective discussions on the current state of knowledge of sperm chromatin structure and function during fertilization. It highlights the biological, assay and clinical uncertainties that require further research and ends with a series of 5 key recommendations.
本文报道了 EHSRE 系列研讨会中最新的一次研讨会的结果，旨在综合当前男性学领域的研究现状，并为未来的工作提供建议（详细信息见附录）。其重点是检测精子 DNA 损伤的方法，以及关于精子染色质组织、易损性和修复的新知识的潜在应用，以改善与这种损伤相关的临床不育症的诊断和治疗。同样重要的是这些测试的使用和可靠性，以确定环境污染物或药物制剂在多大程度上可能导致精子 DNA 损伤和男性生育问题的发生。一个在 ESHRE 支持下的工作组于 2009 年 5 月召开会议，评估了当前的知识库，并提出了未来基础和临床研究的方向。本文综合了工作组对精子染色质结构和功能受精过程中当前知识的理解，以及对最近文献的集体讨论。它强调了需要进一步研究的生物学、检测和临床不确定性，并以一系列 5 项关键建议结束。
