Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India.
Biotechnol Lett. 2010 May;32(5):707-12. doi: 10.1007/s10529-010-0211-2. Epub 2010 Feb 6.
A quick multiplex PCR based detection method was developed for early diagnosis of typhoid using specific genetic markers of S. typhi. Primers of tyv gene, flag gene, viaB gene and ratA gene confirmed the specificity and sensitivity of the PCR. The serum samples of the suspected typhoid patients were taken directly for PCR without culturing and isolating genomic DNA. Overall diagnosis required 2 h which is the least time ever reported for a PCR based methods. The sensitivity of the method is up to 5 fg genomic DNA. The genetic markers are specific and the four pairs of primers give selective amplification and differentiate S. typhi from closely related S. typhimurium.
建立了一种快速多重 PCR 检测方法，用于使用伤寒沙门氏菌的特定遗传标记物进行伤寒的早期诊断。tyv 基因、鞭毛基因、viaB 基因和 ratA 基因的引物证实了 PCR 的特异性和敏感性。疑似伤寒患者的血清样本无需培养和分离基因组 DNA 即可直接进行 PCR。总体诊断需要 2 小时，这是基于 PCR 的方法中报告的最短时间。该方法的灵敏度高达 5 fg 基因组 DNA。遗传标记物是特异性的，四对引物进行选择性扩增，可将伤寒沙门氏菌与密切相关的鼠伤寒沙门氏菌区分开来。
