Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, 410001 Enugu State, Nigeria.
Nat Prod Res. 2010 Feb;24(3):266-73. doi: 10.1080/14786410902986894.
Alchornea floribunda leaves are widely used in African ethnomedicine for the management of acute and chronic inflammatory disorders. In the present study, bioactivity-guided fractionation of the ethyl acetate fraction of the methanol leaf extract of the plant material led to the isolation of a new flavonol glycoside (AFF1). The anti-inflammatory activity of this novel compound was evaluated using egg albumen-induced rat paw oedema as a model of inflammation. AFF1 showed significant inhibition of the rat paw oedema in a dose-dependent manner. The activity of AFF1 (50 mg kg(-1)) was higher than that of the standard anti-inflammatory drug, aspirin (100 mg kg(-1)). The compound also significantly (p < 0.001) inhibited heat-induced haemolysis of human erythrocytes in vitro. The structure of AFF1 was elucidated as 3,5,7,3'-tetrahydroxyflavone-3-O-alpha-L-rhamnoside, using a combination of UV, IR, 1D and 2D (COSY) 1H-NMR spectroscopy. This compound, in part, accounts for the anti-inflammatory effect of A. floribunda leaves.
阿尔采末尼亚弗洛里邦达叶广泛应用于非洲民族医学，用于治疗急性和慢性炎症性疾病。在本研究中，对植物材料甲醇叶提取物的乙酸乙酯部分进行生物活性导向分离，得到了一个新的类黄酮糖苷（AFF1）。该新型化合物的抗炎活性采用蛋清诱导的大鼠足肿胀模型进行评估。AFF1 以剂量依赖的方式显著抑制大鼠足肿胀。AFF1（50mg/kg）的活性高于标准抗炎药物阿司匹林（100mg/kg）。该化合物还显著（p<0.001）抑制体外热诱导的人红细胞溶血。使用 UV、IR、1D 和 2D（COSY）1H-NMR 光谱的组合，阐明了 AFF1 的结构为 3,5,7,3'-四羟基黄酮-3-O-α-L-鼠李糖苷。该化合物部分解释了阿尔采末尼亚弗洛里邦达叶的抗炎作用。
