阿尔楚尼叶中的一种新的抗炎类黄酮糖苷。

A new anti-inflammatory flavonol glycoside from Alchornea floribunda leaves.

机构信息

Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, 410001 Enugu State, Nigeria.

出版信息

Nat Prod Res. 2010 Feb;24(3):266-73. doi: 10.1080/14786410902986894.

DOI:10.1080/14786410902986894

PMID:20140805

Abstract

Alchornea floribunda leaves are widely used in African ethnomedicine for the management of acute and chronic inflammatory disorders. In the present study, bioactivity-guided fractionation of the ethyl acetate fraction of the methanol leaf extract of the plant material led to the isolation of a new flavonol glycoside (AFF1). The anti-inflammatory activity of this novel compound was evaluated using egg albumen-induced rat paw oedema as a model of inflammation. AFF1 showed significant inhibition of the rat paw oedema in a dose-dependent manner. The activity of AFF1 (50 mg kg(-1)) was higher than that of the standard anti-inflammatory drug, aspirin (100 mg kg(-1)). The compound also significantly (p < 0.001) inhibited heat-induced haemolysis of human erythrocytes in vitro. The structure of AFF1 was elucidated as 3,5,7,3'-tetrahydroxyflavone-3-O-alpha-L-rhamnoside, using a combination of UV, IR, 1D and 2D (COSY) 1H-NMR spectroscopy. This compound, in part, accounts for the anti-inflammatory effect of A. floribunda leaves.

摘要

阿尔采末尼亚弗洛里邦达叶广泛应用于非洲民族医学，用于治疗急性和慢性炎症性疾病。在本研究中，对植物材料甲醇叶提取物的乙酸乙酯部分进行生物活性导向分离，得到了一个新的类黄酮糖苷（AFF1）。该新型化合物的抗炎活性采用蛋清诱导的大鼠足肿胀模型进行评估。AFF1 以剂量依赖的方式显著抑制大鼠足肿胀。AFF1（50mg/kg）的活性高于标准抗炎药物阿司匹林（100mg/kg）。该化合物还显著（p<0.001）抑制体外热诱导的人红细胞溶血。使用 UV、IR、1D 和 2D（COSY）1H-NMR 光谱的组合，阐明了 AFF1 的结构为 3,5,7,3'-四羟基黄酮-3-O-α-L-鼠李糖苷。该化合物部分解释了阿尔采末尼亚弗洛里邦达叶的抗炎作用。