College of Pharmacy, IPS Academy, Indore, MP, India.
Nat Prod Res. 2010 Feb;24(3):300-4. doi: 10.1080/14786410903237123.
We evaluate the in vitro free radical scavenging activity of the leaves of Tabernaemontana divaricata Linn. Petroleum ether, ethanol and aqueous extracts of T. divaricata were prepared with successive extraction in a soxhlet apparatus. Each extract was selected to study the free radical scavenging activity by superoxide scavenging assay method. It was found that the aqueous extract contained carbohydrates, glycosides, amino acids, flavonoids, tannins, alkaloids, and steroids, and the ethanolic extract contained glycosides, amino acids, flavonoids, tannins, alkaloids and steroids. The ethanolic extract of T. divaricata showed 58.7 +/- 0.62% inhibition in the superoxide scavenging model. The aqueous extract also showed almost similar activity (54.9 +/- 0.53% compared to the ethanolic extract), while petroleum ether extract showed poor inhibition of superoxide scavenging activity. All extracts showed the dose- and time-dependent inhibition of the superoxide scavenging activity.
我们评估了 Tabernaemontana divaricata Linn. 叶的体外自由基清除活性。采用索氏提取器连续提取，制备了 Tabernaemontana divaricata 的石油醚、乙醇和水提取物。选择每种提取物通过超氧阴离子清除测定法来研究自由基清除活性。结果发现，水提取物含有碳水化合物、糖苷、氨基酸、类黄酮、单宁、生物碱和类固醇，而乙醇提取物含有糖苷、氨基酸、类黄酮、单宁、生物碱和类固醇。Tabernaemontana divaricata 的乙醇提取物在超氧阴离子清除模型中显示出 58.7 +/- 0.62%的抑制作用。水提取物也表现出几乎相似的活性（与乙醇提取物相比为 54.9 +/- 0.53%），而石油醚提取物对超氧阴离子清除活性的抑制作用较差。所有提取物均表现出剂量和时间依赖性的超氧阴离子清除活性抑制作用。
