Department of Pharmacognosy, Herbal Drug Discovery Division, BRNSS-Contract Research Center-SIRO, B.R. Nahata College of Pharmacy, Mandsaur 458001, Madhya Pradesh, India.
Nat Prod Res. 2010 May;24(9):855-60. doi: 10.1080/14786410903314385.
The aim of the present study was to assess the anti-fertility activity of ethanolic extracts of Tabernaemontana divaricata (TD) leaves in oestrogenic activity models in immature female rats. Mature green leaves of TD were collected and authenticated. Extractions of the dried leaves were carried out with ethanol in a Soxhlet's apparatus. For oestrogenic activity, the extracts were administered orally once daily at a dose of 200 and 400 mg kg(-1), and the activity was compared with the standard drug ethinyl oestradiol (0.02 mg). The extracts caused significant increase in uterine weight compared to the control. The ethanolic extract exhibited oestrogenic activity. The histological study of epithelium tissues with the 400 mg of TD extract-treated animals showed increases in the height of the luminal epithelium and loose edematous stroma when compared with the 200 mg of TD extract-treated group of animals. However, this was better than the control group of animals. Enhanced uterine weight and increase in the height of luminal epithelium and histological characteristics suggest that TD extract may be useful in anti-fertility therapy.
本研究旨在评估 Tabernaemontana divaricata（TD）叶的乙醇提取物在未成年雌性大鼠的雌激素活性模型中的抗生育活性。采集成熟的 TD 绿叶并进行鉴定。用索氏提取器中的乙醇对干燥的叶子进行提取。对于雌激素活性，提取物以 200 和 400mg/kg(-1)的剂量每天口服一次，并将活性与标准药物乙炔雌二醇（0.02mg）进行比较。与对照组相比，提取物导致子宫重量显著增加。乙醇提取物表现出雌激素活性。用 400mg TD 提取物处理的上皮组织的组织学研究表明，与用 200mg TD 提取物处理的动物组相比，腔上皮的高度增加和疏松水肿的基质增加。然而，这比对照组的动物要好。增强的子宫重量和腔上皮高度的增加以及组织学特征表明，TD 提取物可能在抗生育治疗中有用。
