College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012.
College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012.
Chin J Nat Med. 2013 Sep;11(5):472-6. doi: 10.1016/S1875-5364(13)60086-2.
To study the in vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice.
Aqueous decoction and methanol leaf extracts were tested for their ability to reduce croton oil-induced edema in the mouse ear after topical application. The methanol leaf extract dose-dependently inhibited the croton oil-induced ear edema in mice (ID50 <500 μg·cm(-2)). A bioassay-guided liquid-liquid fractionation of this methanol extract gave four active fractions: water insoluble (F1), hexane (F2), ethyl acetate (F3) and water (F4).
The hexane fraction showed a very high activity (42.1% inhibition at 0.7 μg·cm(-2)) as compared to the control. The other fractions were less active (F1: 56.1% at 506.2 μg·cm(-2); F3: 57.3% at 289.3 μg·cm(-2); and F4: 31.9% for 203.8 μg·cm(-2)) while indomethacin gave 48.8% of inhibition at 90 μg·cm(-2). The activity of F1 and F3 may be at least in part explained by the presence of anti-inflammatory flavonoids, while the activity was not correlated to the tannin contents. No compounds were detected in the most active F2 fraction.
The results give a rational support to the traditional use of T. divaricata in tropical India as anti-inflammatory agent.
研究鸡屎树叶提取物对雄性白化小鼠体内抗炎活性。
通过局部应用评估水提物和甲醇叶提取物对减轻小鼠耳中巴豆油诱导水肿的能力。甲醇叶提取物剂量依赖性地抑制了小鼠耳中巴豆油诱导的水肿（ID50<500μg·cm(-2)）。该甲醇提取物的生物测定指导的液-液分馏给出了四个活性馏分：水不溶性（F1）、己烷（F2）、乙酸乙酯（F3）和水（F4）。
与对照组相比，己烷馏分表现出非常高的活性（0.7μg·cm(-2)时抑制率为 42.1%）。其他馏分活性较低（F1：在 506.2μg·cm(-2)时抑制率为 56.1%；F3：在 289.3μg·cm(-2)时抑制率为 57.3%；F4：在 203.8μg·cm(-2)时抑制率为 31.9%），而吲哚美辛在 90μg·cm(-2)时抑制率为 48.8%。F1 和 F3 的活性至少部分归因于抗炎黄酮类化合物的存在，而活性与单宁含量无关。最活跃的 F2 馏分中未检测到任何化合物。
这些结果为鸡屎树在印度热带地区作为抗炎剂的传统用途提供了合理的支持。
