Department of Pharmacology, SCS College of Pharmacy, Harapanahalli, Davanagere (District), Karnataka, India.
Chin J Nat Med. 2012 Jan;10(1):58-62. doi: 10.1016/S1875-5364(12)60013-2.
To evaluate the anti-fertility effect of methanolic (MeTD) and aqueous (AqTD) flower extracts of Tabernaemontana divaricata in rats.
The anti-fertility activity of the extracts was evaluated using two experimental animal models: 1) Estrogenic activity was carried out in immature female rats using ethinyl estradiol as standard. The evaluation parameters includes changes in uterine weight and histopathology of uterus. 2) Anti-implantation and early abortifacient activity was performed in female Wistar rats. The number of implants and resorbtions were compared to vehicle control.
Phytochemical analysis of MeTD and AqTD revealed the presence of carbohydrates, amino acids, steroids, glycosides, flavonoids, alkaloids and tannins. In estrogenic activity, the MeTD and AqTD were offered significant estrogen-like activity at 500 mg·kg(-1), p.o. by increasing the uterine weight compared to vehicle control group. In Anti-implantation and early abortifacient activity study, MeTD (500 mg·kg(-1), p.o.) showed significant effect and it was evident by decrease in the number of implants and increase in the number of resorbtions compared to vehicle control group.
The MeTD at 500 mg·kg(-1), p.o. possess significant estrogenic, anti-implantation and early abortifacient activity, while the AqTD at 500 mg·kg(-1), p.o. was found to possess significant estrogenic activity and the results are in consistent with the literature reports related to anti-fertility effect of flower extracts of Tabernaemontana divaricata.
评估黄蝉 Tabernaemontana divaricata 的甲醇（MeTD）和水（AqTD）花提取物对大鼠的抗生育作用。
使用两种实验动物模型评估提取物的抗生育活性：1）使用乙炔雌二醇作为标准，在未成年雌性大鼠中进行雌激素活性评估。评估参数包括子宫重量的变化和子宫组织病理学变化。2）在雌性 Wistar 大鼠中进行抗着床和早期流产活性。与载体对照组相比，比较植入物和吸收的数量。
MeTD 和 AqTD 的植物化学分析显示存在碳水化合物、氨基酸、类固醇、糖苷、类黄酮、生物碱和单宁。在雌激素活性中，MeTD 和 AqTD 在 500 mg·kg(-1)，p.o. 时表现出显著的雌激素样活性，与载体对照组相比，增加了子宫重量。在抗着床和早期流产活性研究中，MeTD（500 mg·kg(-1)，p.o.）表现出显著的效果，与载体对照组相比，植入物数量减少，吸收数量增加。
MeTD 在 500 mg·kg(-1)，p.o. 时具有显著的雌激素、抗着床和早期流产活性，而 AqTD 在 500 mg·kg(-1)，p.o. 时具有显著的雌激素活性，结果与黄蝉花提取物的抗生育作用的文献报道一致。
