Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
Langmuir. 2010 Feb 16;26(4):2924-9. doi: 10.1021/la902683x.
Herein, a functional template made up of in situ synthesized silver nanoparticles (AgNPs) is prepared on polydimethylsiloxane (PDMS) for the spatial control of cell capture, where the residual Si-H groups in the PDMS matrix are used as reductants to reduce AgNO(3) for forming AgNPs. In virtue of microfluidic system, a one-dimensional array pattern of AgNPs is obtained easily. Further combining with plasma treatment, a two-dimensional array pattern of AgNPs could be achieved. The obtained PDMS-AgNPs composite is characterized in detail. The PDMS-AgNPs composite shows good antibacterial property in E. coli adhesion tests. The patterns possess hifi and high resolution (ca. 8 microm). Cell patterns with high efficiency and spatial selectivity are further formed with the aid of H-Arg-Gly-Asp-Cys-OH (RGDC) tetrapeptide which is grafted on the AgNPs template. Cells immobilized on the template show a good ability for adhesion, spreading, migration, and growth.
本文在聚二甲基硅氧烷（PDMS）上制备了一种由原位合成的银纳米粒子（AgNPs）组成的功能模板，用于空间控制细胞捕获，其中 PDMS 基质中的残留 Si-H 基团可用作还原剂来还原 AgNO3 以形成 AgNPs。借助微流控系统，很容易获得一维 AgNPs 阵列图案。进一步结合等离子体处理，可以获得二维 AgNPs 阵列图案。详细表征了所得的 PDMS-AgNPs 复合材料。在大肠杆菌黏附实验中，PDMS-AgNPs 复合材料表现出良好的抗菌性能。图案具有高清晰度和高分辨率（约 8 微米）。借助接枝在 AgNPs 模板上的 H-Arg-Gly-Asp-Cys-OH（RGDC）四肽，进一步形成了具有高效和空间选择性的细胞图案。固定在模板上的细胞表现出良好的黏附、扩展、迁移和生长能力。
