Kishen Anil, Shrestha Annie, Rafique Adeela
Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore, Republic of Singapore, Singapore.
J Conserv Dent. 2008 Apr;11(2):63-70. doi: 10.4103/0972-0707.44053.
In this study, a Fiber Optic Backscatter Spectroscopic Sensor (FOBSS) is used to monitor demineralization and remineralization induced changes in the enamel. A bifurcated fiber optic backscatter probe connected to a visible light source and a high resolution spectrophotometer was used to acquire the backscatter light spectrum from the tooth surface. The experiments were conducted in two parts. In Part 1, experiments were carried out using fiber optic backscatter spectroscopy on (1) sound enamel and dentine sections and (2) sound tooth specimens subjected to demineralization and remineralization. In Part 2, polarization microscopy was conducted to examine the depth of demineralization in tooth specimens. The enamel and dentine specimens from the Part-1 experiments showed distinct backscatter spectra. The spectrum obtained from the enamel-dentine combination and the spectrum generated from the average of the enamel and dentine spectral values were closely similar and showed characteristics of dentine. The experiments in Part 2 showed that demineralization and remineralization processes induced a linear decrease and linear increase in the backscatter light intensity respectively. A negative correlation between the decrease in the backscatter light intensity during demineralization and the depth of demineralization determined using the polarization microscopy was calculated to be p = -0.994. This in vitro experiment highlights the potential benefit of using FOBSS to detect demineralization and remineralization of enamel.
在本研究中，使用光纤背散射光谱传感器（FOBSS）来监测牙釉质中脱矿和再矿化引起的变化。一个连接到可见光源和高分辨率分光光度计的分叉光纤背散射探头用于获取牙齿表面的背散射光谱。实验分两部分进行。在第1部分中，使用光纤背散射光谱对（1）完好的牙釉质和牙本质切片以及（2）经过脱矿和再矿化的完好牙齿标本进行实验。在第2部分中，进行偏振显微镜检查以观察牙齿标本中的脱矿深度。第1部分实验中的牙釉质和牙本质标本显示出明显的背散射光谱。从牙釉质 - 牙本质组合获得的光谱以及由牙釉质和牙本质光谱值的平均值生成的光谱非常相似，并显示出牙本质的特征。第2部分的实验表明，脱矿和再矿化过程分别导致背散射光强度呈线性下降和线性增加。经计算，脱矿过程中背散射光强度的下降与使用偏振显微镜确定的脱矿深度之间的负相关系数为p = -0.994。这项体外实验突出了使用FOBSS检测牙釉质脱矿和再矿化的潜在益处。
