Pretty I A, Edgar W M, Higham S M
Department of Clinical Dental Sciences, The University of Liverpool, Edwards Building, Daulby Street, L69 3GN, Liverpool, UK.
J Dent. 2003 Jul;31(5):313-9. doi: 10.1016/s0300-5712(03)00067-8.
Longitudinal in vitro.
Previously extracted, caries free, human premolars were selected and prepared by gentle pumicing and coating in an acid-resistant nail-varnish save for an exposed enamel window on the buccal surface. Each was assigned to one of eight groups (six per group, 10 in positive control); positive control (citric acid, pH 2.7, F(-) 0 ppm), negative control (pH 7.0, F(-) 0 ppm) Listerine (pH 3.87, F(-) 0.021 ppm), Tesco Value (pH 6.05, F(-) 289.00 ppm), Tesco Total Care (pH 6.20, F(-) 313.84 ppm), Sainsbury's (pH 6.15, F(-) 365.75 ppm), Sensodyne (pH 6.12, F(-) 285.30 ppm) and Corsodyl (pH 5.65, F(-) 0 ppm). The titratable acid values (TAV) for each rinse were established using volume (ml) of 0.1 M NaOH to achieve pH 7. Fluoride values were obtained by ion selective electrode. The solutions were kept at 37 degrees C and gently agitated. Teeth were removed at hourly intervals for 15 h, air-dried and subjected to Quantitative Light-induced Fluorescence (QLF) examination by a blinded examiner and DeltaQ values recorded. At the conclusion of the study each of the positive control teeth and one from each other group were sectioned through the eroded lesion, ground and polished to 100 micrometers and subjected to transverse microradiography and DeltaZ recorded for validation.
TAVs were: Listerine 2.45 L > Sainsbury's 0.35 ml >Tesco Total Care 0.14 ml > Tesco Value 0.08 ml > Corsodyl 0.10 ml >Sensodyne 0.9 ml. DeltaQ increased over time for the positive control, (0 h 0.2, 10 h 95.2, 15 h 152.3). Negative controls remained stable. The increase in DeltaQ for each rinse after 15 h was Listerine (9.3(+/-7.2)), Corsodyl (1.5(+/-1.2)), Tesco Value (1.8(+/-1.2)), Tesco Total Care (1.4(+/-1.1)), Sainsbury's (3.4(+/-2.2)), Sensodyne (0.9(+/-1.6)). TMR confirmed the presence/absence of erosive lesions.
QLF effectively monitored erosion in the positive controls and lack of erosion in the NC. Only one mouthrinse (Listerine) caused any erosion compared to the negative control, but this was only significant after 14 h of continuous use.
纵向体外研究。
选取先前拔除的、无龋的人类前磨牙，通过轻柔打磨并用耐酸指甲油涂层处理，仅保留颊面暴露的釉质窗口。将每颗牙齿分配到八组中的一组（每组六颗，阳性对照组十颗）；阳性对照（柠檬酸，pH 2.7，氟离子浓度0 ppm），阴性对照（pH 7.0，氟离子浓度0 ppm），李施德林漱口水（pH 3.87，氟离子浓度0.021 ppm），乐购超值漱口水（pH 6.05，氟离子浓度289.00 ppm），乐购全护漱口水（pH 6.20，氟离子浓度313.84 ppm），森宝利漱口水（pH 6.15，氟离子浓度365.75 ppm），舒适达漱口水（pH 6.12，氟离子浓度285.30 ppm）和高露洁漱口水（pH 5.65，氟离子浓度0 ppm）。使用0.1 M氢氧化钠的体积（毫升）来确定每种漱口水的可滴定酸值（TAV），以达到pH 7。通过离子选择性电极获得氟离子浓度值。溶液保持在37摄氏度并轻轻搅拌。每隔一小时取出牙齿，持续15小时，空气干燥后由一位不知情的检查者进行定量光诱导荧光（QLF）检查并记录DeltaQ值。在研究结束时，将每组中的一颗阳性对照牙齿和一颗其他组牙齿沿侵蚀病变处切开，研磨并抛光至100微米，进行横向显微放射摄影并记录DeltaZ值以进行验证。
可滴定酸值（TAV）分别为：李施德林漱口水2.45毫升＞森宝利漱口水0.35毫升＞乐购全护漱口水0.14毫升＞乐购超值漱口水0.08毫升＞高露洁漱口水0.10毫升＞舒适达漱口水0.9毫升。阳性对照的DeltaQ值随时间增加（0小时0.2，10小时95.2，15小时152.3）。阴性对照保持稳定。15小时后，每种漱口水的DeltaQ值增加情况为：李施德林漱口水（9.3（±7.2）），高露洁漱口水（1.5（±1.2）），乐购超值漱口水（1.8（±1.2）），乐购全护漱口水（1.4（±1.1）），森宝利漱口水（3.4（±2.2）），舒适达漱口水（0.9（±1.6））。横向显微放射摄影证实了侵蚀性病变的存在与否。
QLF有效地监测了阳性对照中的侵蚀情况以及阴性对照中无侵蚀的情况。与阴性对照相比，只有一种漱口水（李施德林漱口水）导致了任何侵蚀，但这仅在连续使用14小时后才显著。
