Kishen A, Chen N N, Tan L, Asundi A
Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore.
J Endod. 2004 Dec;30(12):872-5. doi: 10.1097/01.don.0000129038.97791.8a.
In this study, optical spectroscopy was used to monitor a chromogenic, enzyme-substrate reaction for the rapid identification of Enterococcus faecalis. The detection system, comprising a miniature spectrophotometer and an accompanying data acquisition system, was placed in an incubator. During testing, a 3-ml test sample was placed in a cuvette within the spectrophotometer. This permitted online, real-time, and remote analysis of spectral signature needed to monitor the bacteria. It was observed that the absorption peak intensity increased conspicuously 3.5 h after inoculation and through the entire period of testing. A linear-regression analysis demonstrated a significant correlation between the increase in absorption peak intensity at 610 nm (r = 0.9389) and 653 nm (r = 0.9387) with the formation of colony-forming units. Optical spectroscopy-based sensing systems can pave the way for rapid, nonlaboratory-based approaches to monitor microbial status quantitatively and qualitatively from clinical samples.
在本研究中，利用光学光谱法监测一种用于快速鉴定粪肠球菌的显色酶底物反应。该检测系统由一台微型分光光度计和一个配套的数据采集系统组成，放置在培养箱中。测试期间，将3毫升测试样品置于分光光度计内的比色皿中。这使得能够对监测细菌所需的光谱特征进行在线、实时和远程分析。观察到接种后3.5小时及整个测试期间，吸收峰强度显著增加。线性回归分析表明，610纳米（r = 0.9389）和653纳米（r = 0.9387）处吸收峰强度的增加与菌落形成单位的形成之间存在显著相关性。基于光学光谱的传感系统可为从临床样本中定量和定性监测微生物状态的快速、非实验室方法铺平道路。
