National Centre for Adult Stem Cell Research, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Nathan 4111, Brisbane, QLD, Australia.
Cell Mol Life Sci. 2010 May;67(10):1735-50. doi: 10.1007/s00018-010-0280-3. Epub 2010 Feb 9.
The growth and guidance of primary olfactory axons are partly attributed to the presence of olfactory ensheathing cells (OECs). However, little is understood about the differences between the subpopulations of OECs and what regulates their interactions. We used OEC-axon assays and determined that axons respond differently to peripheral and central OECs. We then further purified OECs from anatomically distinct regions of the olfactory bulb. Cell behaviour assays revealed that OECs from the olfactory bulb were a functionally heterogeneous population with distinct differences which is consistent with their proposed roles in vivo. We found that the heterogeneity was regulated by motile lamellipodial waves along the shaft of the OECs and that inhibition of lamellipodial wave activity via Mek1 abolished the ability of the cells to distinguish between each other. These results demonstrate that OECs from the olfactory bulb are a heterogeneous population that use lamellipodial waves to regulate cell-cell recognition.
初级嗅神经轴突的生长和引导部分归因于嗅鞘细胞（OEC）的存在。然而，对于 OEC 亚群之间的差异以及调节它们相互作用的因素知之甚少。我们使用 OEC-轴突测定法，发现轴突对周围和中枢 OEC 的反应不同。然后，我们进一步从嗅球的解剖学上不同区域纯化 OEC。细胞行为测定表明，来自嗅球的 OEC 是一个功能异质的群体，具有明显的差异，这与其在体内的预期作用一致。我们发现，这种异质性受 OEC 轴突上的运动片状伪足波调节，并且通过 Mek1 抑制片状伪足波活性会使细胞丧失彼此区分的能力。这些结果表明，来自嗅球的 OEC 是一个异质群体，它使用片状伪足波来调节细胞间识别。
